Promega Corporation

CytoTox 96® Non-Radioactive Cytotoxicity Assay

The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96® Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected usi...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega KK only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

CytoTox 96® Non-Radioactive Cytotoxicity Assay

Close Window

CytoTox 96® Non-Radioactive Cytotoxicity Assay

 Components

  • Substrate Mix

    G179A5 x 1.5ml
  • Assay Buffer

    G180A1 x 60ml
  • LDH Positive Control

    G181A1 x 25μl
  • Lysis Solution, 10X

    G182A1 x 5ml
  • Stop Solution

    G183A1 x 65ml
1,000 assays
- G1780 Please Enquire


You Also May Be Interested In

Storage Conditions

Store Substrate Mix and Assay Buffer at –20°C. Store LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

G1780 For Research Use Only. Not for Use in Diagnostic Procedures.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.