AMP-Glo™ Assay

V5011_AMP-Glo--Assay--1000-Assays_3
customize-this-small
View information on global supply logistics

Monitor AMP Concentration in Biochemical Reactions

  • Use with virtually with any AMP-producing enzyme
  • Well-suited for low-activity enzymes
  • Sensitive to low AMP concentration

Size

Catalog number selected: V5011

$ 820.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
AMP-Glo™ Assay
1,000 assays
$ 820.00
Your price: Log in
Change Configuration

Sensitive, Luminescent AMP Detection 

The AMP-Glo™ Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP.  It can be used to measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. 

The assay quantitatively monitors AMP concentration in a biochemical reaction in a wide range of plates, including high-throughput formats. The stable luminescent signal eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate.

Two reagents are supplied: one to terminate the AMP-generating enzymatic reaction, remove ATP and convert AMP produced into ADP, and a second reagent to convert ADP to ATP, which is used to generate a luminescence in a luciferase reaction. The AMP-Glo™ Assay is well suited for monitoring AMP produced in biochemical reactions catalyzed by enzymes that do not use ATP as a substrate, such as cAMP-dependent phosphodiesterases (PDE) and bacterial DNA ligases.

AMP-Glo™ Assay principle.

Screen Library Compounds for Effects on AMP-Producing Enzymes 

The AMP-Glo™ Assay has a high dynamic range and produces a strong signal at low substrate conversion, allowing screening of low-activity enzymes. The assay produces minimal false hits and Z´ values greater than 0.7, and can be used to screen library compounds for effects on target enzymes such as:

  • ubiquitin ligases
  • cyclic AMP-specific phosphodiesterases
  • aminoacyl-tRNA synthetases
  • DNA ligases
  • poly(A) deadenylases
  • demethylases (indirect method)

AMP-Glo™ Assays contain enough reagent to perform the indicated number of reactions in a low-volume 384-well plate format using 5μl of reaction enzyme, 5μl of AMP-Glo™ I Reagent and 10μl of AMP Detection Solution. The system also can be used in standard 96-well plates (25μl:25μl:50μl).

See More Data: This peer-reviewed paper demonstrates how the AMP-Glo™ Assay was used to monitor and characterize soluble and membrane-bound ectonucleotidases CD73 and CD39.

Read the Paper
60109223-ampglo-paper-image-1030x1450

Specifications

You are viewing: V5011 Change Configuration

What's in the box?

Item Part # Size
AMP-Glo™ Reagent I V503A 1 × 5ml
AMP-Glo™ Reagent II V504A 1 × 100μl
Kinase-Glo® One Solution V505A 1 × 10ml
AMP, 10mM V506A 1 × 100μl

Ultra Pure ATP, 10mM

V915A 1 × 500μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5012 Change Configuration

What's in the box?

Item Part # Size
AMP-Glo™ Reagent I V503B 1 × 50ml
AMP-Glo™ Reagent II V504B 1 × 1ml
Kinase-Glo® One Solution V505B 1 × 100ml
AMP, 10mM V506B 1 × 1ml

Ultra Pure ATP, 10mM

V915B 1 × 5ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-angus

Angus

UK