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Bacterial Strain JM109(DE3)

Part Numbers: P9801

P9801_Bacterial Strain JM109_DE3
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T7 プロモーターによる遺伝子高レベル発現に

  • IPTGで誘導されるT7 RNAポリメラーゼ遺伝子を持つ
  • リボゾーム結合配列がクローン配列中に必要
  • 青/白スクリーニングはできない

Size

選択製品のカタログ番号: P9801

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This product is discontinued
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Bacterial Strain JM109(DE3)
500μl
Your price:

JM109菌株に由来する本菌株は、転写活性が強化されたlacプロモーターに制御されたT7 RNAポリメラーゼ遺伝子のコピーを持つλファージDE3が染色体上に溶原化しています。T7 プロモーターをもつFlexi® VectorやpGEM® Vectorの宿主として、リボソーム結合部位を有するクローン化DNAを高いレベルで発現させることが可能となります。JM109(DE3)菌株は青/白スクリーニングに使用できません。

Genotype:  endA1 recA1 gyrA96 thi-1 hsdR17 (rK–, mK+) relA1 supE44 Δ(lac-proAB) λ(DE3) /  F' [traD36 proA+B+ lacIq lacZΔM15]

 

  • T7プロモーター発現: T7 RNAポリメラーゼのIPTG誘導遺伝子が含まれています。
  •  in vivoでの融合タンパク質の合成
【カルタヘナ】対象品

製品仕様

選択製品の構成品内容

Item Part # Size

Bacterial Strain JM109(DE3)

 P980A 1 × 500μl

SDS

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分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

AA

技術資料

サイテーション

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高い形質転換効率とタンパク質発現制御を兼ね備えた組換えタンパク質発現に最適なE. coli 株

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様々な細菌におけるβ-ガラクトシダーゼ活性の誘導剤

V3955, V3951, V3953

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