RNase ONE™ Ribonuclease
Degrades RNA to Cyclic Nucleotide Monophosphate (NMP) Intermediates
- Ability to cleave a phosphodiester bond between any two ribonucleotides
- Supplied at a concentration of 5–10u/μl
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
Catalog Number: M4261
Catalog Number: M4265
- 柔軟性: RNase ONE™ Ribonuclease は、2つのリボヌクレオチド間のホスホジエステラーゼ結合を切断することが可能です。
- 10X Reaction Buffer添付: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate.
- 一本鎖領域に対する選択的切断を用いたRNAマッピングあるいは定量
- RNaseプロテクションアッセイ
- ミスマッチの検出
- 調製したDNAからRNAを選択的に除去
さらに知りたい方は、プロトコール＆ガイド(英語) をご覧ください。
- Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.
- Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187, 549–53.
- Meador, J., 3rd and Kennell, D. (1990) Gene 95, 1–7.
Storage Buffer: 10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase.
Unit Definition: One unit is defined as the amount of enzyme required tocompletely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.
Protocols
No protocols available
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
RNase ONE™ 10X Reaction Buffer |
M217A | 1 × 1ml |
|
RNase ONE™ Ribonuclease |
M426A | 1 × 1,000u |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
See Protocol for storage recommendations.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
RNase ONE™ 10X Reaction Buffer |
M217A | 5 × 1ml |
|
RNase ONE™ Ribonuclease |
M426C | 1 × 5,000u |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
See Protocol for storage recommendations.
Resources
No related resources available
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