M6101_RQ1-RNase-Free-DNase--1-000u_3

Degrades Single-Stranded or Double-Stranded DNA

  • Produces 3´ hydroxyl oligonucleotides during degradation
  • Preparation qualified for use in applications where maintaining the integrity of RNA is critical
  • Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated

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Catalog Number: M6101

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RQ1 RNase-Free DNaseは、高純度に精製されたDNase I 製品で、一本鎖あるいは二本鎖DNAを分解して3'-末端に水酸基を持つオリゴヌクレオチドを生成します。特にRNAの保護が必須である実験系で使用されます。RNase活性が全く検出されないことを、酵素処理後のRNA、DNA混合溶液のゲル解析法を含む2種類の方法で確認しています。

  • 10X Reaction Buffer 添付:400mM Tris-HCl (pH8.0 at25°C), 100mM MgSO4, 10mM CaCl2
  • Stop Buffer 添付:20mM EGTA (pH8.0 at 25°C)

  • DNA混入の無いRNA調製
  • RNA転写システムにおけるDNAの分解
  • DNAのニックトランスレーション
  • DNase I フットプリンティング法を用いたDNA-タンパク質相互作用の研究

【Q&A】RQ1 RNase-Free DNase

Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.

Source: Bovine pancreas.

QC Tests: Activity, RNase.

Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.

Specifications

Catalog Number:

選択製品の構成品内容

Item Part # Size Available Separately

RQ1 DNase 10X Reaction Buffer

M198A 1 × 1ml

RQ1 DNase Stop Solution

M199A 1 × 1ml

RQ1 RNase-Free DNase

M610A 1 × 1,000u View Product

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使用制限

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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