M3011_Single-Stranded-DNA-Binding-Protein--100_3

Cooperatively Binds ssDNA with High Affinity

  • Consists of four identical 18.9kDa subunits
  • Does not bind well to double-stranded DNA
  • Supplied at a concentration of 1–5µg/μl

Catalog Number:

Size

Catalog Number: M3011

Please Enquire


Overview
Protocols
Specifications
Resources
Related Products
E.coli のSingle-Stranded DNA Binding Protein (SSB)は、分子量18.9kDaの単一サブユニットから構成される四量体です。一本鎖DNAとの親和性が高く、SSBが協調的に結合しますが、二本鎖DNAにはほとんど結合しません。in vivo のDNA複製や組換えに関与するタンパク質です。
  • DNAの複製や組換えの研究
  • 隣接するオリゴヌクレオチドを用いたプライマーウォーキング法によるDNAシークエンシング

  1. Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4274–8.
  2. Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55, 103–36.
  3. Krauss, G. et al. (1981) Biochemistry 20, 5346–52.
  4. Weiner, J.H. et al. (1975) J. Biol. Chem. 250, 1972–80.

Storage Buffer: 50mM Tris-HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% (v/v) glycerol.

Source: E. coli.

QC Tests: Protein concentration, DNase, RNase, SDS-PAGE/purity, endonuclease.

Protocols

No protocols available

Specifications

Catalog Number:

選択製品の構成品内容

Item Part # Size

Single-Stranded DNA Binding Protein

M301A 1 × 100μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

Resources

No related resources available

No related products available