T4 DNA Polymerase
Synthesizes DNA 5´→3´ from a Primed ssDNA Template
- High fidelity enzyme of choice for applications where misincorporation is a concern
- Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
- Heat inactivated by heating at 75°C for 10 minutes
- Supplied at a concentration of 5–10u/μl
Catalog Number:
Size
Catalog Number: M4211
Catalog Number: M4215
T4 DNA Polymeraseは、一本鎖DNAテンプレートに結合したプライマーから5'→3'方向にDNAを合成します。強力な3'→5'エキソヌクレアーゼ活性（proofreading）を示しますが、5'→3'エキソヌクレアーゼ活性はありません。
- 高忠実性： 誤取込みが問題となるアプリケーションで最適
- 柔軟性： 様々な分子生物学実験に使用され、多くのプロメガ制限酵素バッファー（1X）中で活性を保ちます
- 熱失活： 75°C、10分間で不活性化します
- T4 DNA Polymerase 10X Buffer 添付：250mM Tris-Acetate (pH7.7), 1M potassium acetate, 100mM magnesium acetate, 10mM DTT
- 標識/未標識dNTPsを用いた5′-付着末端の平滑化
- 3′-付着末端の平滑化
- in vitro 変異導入
【Q&A】T4 DNA Polymerase
- Challberg, M.D. and Englund, P.T. (1980) Meth. Enzymol. 65, 39–43.
- Burd, J.F. and Wells, R.D. (1974) J. Biol. Chem. 249, 7094–801.
- Kunkel, T.A., Loeb, L.A. and Goodman, M.F. (1984) J. Biol. Chem. 259, 1539–45.
Storage Buffer: 200mM potassium phosphate (pH 6.5 at 25°C), 2mM DTT and 50% glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, endonuclease.
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 55nmol of dTTP into acid-precipitable material in 30 minutes at 39°C using poly(dA):oligo(dT) as a substrate.
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
T4 DNA Polymerase |
M421A | 1 × 100u |
|
T4 DNA Polymerase 10X Reaction Buffer |
M831A | 1 × 1ml |
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
T4 DNA Polymerase |
M421F | 1 × 500u |
|
T4 DNA Polymerase 10X Reaction Buffer |
M831A | 1 × 1ml |
Resources
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