Native RNasin® Ribonuclease Inhibitor

Native RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase and Taq DNA polymerases.

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Native RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase and Taq DNA polymerases.

Features - Benefits

• Inhibits Common Eukaryotic RNases: Carries broad-spectrum RNase inhibitory properties.
• Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
• Broad pH Range (pH 5–8): Offers flexibility in downstream assays.

Applications

• RT-PCR.
• Useful in any applications where eukaryotic RNase contamination is a potential problem.
• Protection of mRNA in cDNA synthesis reactions.
• In vitro transcription/translation.
• Increase yield and activity of polysomes.
• Improvement of in vitro virus replication.
• Improvement of RNA translation in homologous systems.
• Performing RNase-free antibody staining.
• Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.

References

1. Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
2. Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
3. Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
4. Lee, F.S. et al. (1989) Biochemistry 28, 225–30.

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