ReliaPrep™ Large Volume HT gDNA Isolation System
Automated DNA Isolation from 1–10ml of Blood
- Effective recovery from pristine, hemolysed or frozen samples
- Purified DNA ready for use in downstream assays or archiving
- Chemistry scaled for each sample, reducing waste
Catalog Number:
Size
Catalog Number: A2751
Scalable, Walkaway DNA Purification from Large-Volume Blood Samples
The ReliaPrep™ Large Volume HT gDNA Isolation System is used with the HSM 3.0 Instrument (Cat.# A2715) to provide a scalable, efficient method for isolating genomic DNA (gDNA) from 1–10ml blood samples. The precipitation-free ReliaPrep™ DNA purification chemistry eliminates the tedious centrifugation steps and hazardous chemicals associated with precipitation-based methods, reducing purification failures. Each kit provides enough reagents to process up to 96 × 10ml whole blood samples.
The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of 1–10ml whole blood, regardless of sample storage or shipping conditions.
Genomic DNA yields from 10ml normal whole blood samples are typically 200–400µg (depending on white blood cell count) in a final eluted volume of 1ml. Recovered DNA exhibits good purity with A260/A280 ratios greater than 1.7 and A260/A230 ratios between 1.8 and 2.2.
Products may not be available in all countries.
Download our Green Sheet to learn more about the features that make this an Environmentally Preferable Product:
Expedite Sample Processing with the HSM 3.0 Heater Shaker Magnet
The HSM 3.0 Instrument heats, shakes and magnetizes sample tubes in one position throughout the extraction process from lysis to elution, eliminating the need to move samples on the robot deck and reducing instrument failures.
For low-throughput isolation of gDNA from up to 32 samples at one time, the HSM 3.0 instrument can be used in a semi-automated mode, where the user performs the pipetting functions. The HSM has software that controls the instrument and directs the user through the purification protocol.
No Manual Sample Transfer Once Instrument Is Loaded
Simply place the primary blood sample tubes on the instrument or transfer samples to processing tubes, follow the setup instructions and start the protocol. Purified DNA is ready in ≤4.5 hours.
Process Multiple Sample Types
| Sample Type | Promega | External | Comments/Reference |
|---|---|---|---|
|
Human Whole Blood
|
Yes
|
No
|
Isolating gDNA from Blood: Simplified gDNA Isolation from Human Blood using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN186, Promega Corporation
|
|
Buffy Coat
|
Yes
|
No
|
Isolating gDNA from Buffy Coat Samples: Semi-Automated or Fully Automated Isolation of gDNA from Buffy Coat Samples Using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN185, Promega Corporation
|
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Alkaline Protease (APA) |
A1721 | 1 × 130ml | View Product |
Cell Lysis Buffer (CLD) |
A1731 | 1 × 1,400ml | View Product |
Binding Buffer (BBA) |
A1741 | 1 × 1,600ml | View Product |
ReliaPrep™ Resin |
A1752 | 1 × 115ml | View Product |
Prepared Wash Buffer (WBC) |
A2681 | 1 × 3,500ml | View Product |
Proteinase K (PK) Solution |
A5051 | 1 × 23ml | View Product |
|
Nuclease-Free Water |
P1195 | 4 × 150ml | View Product |
Related Products
類似製品
関連製品
Heater Shaker Magnet Instrument (HSM 3.0)
大容量の精製法のためのオールインワン処理装置
A2715
QuantiFluor® dsDNA System
二本鎖DNAの特異的、高感度定量システム
E2670, E2671
Quantus™ Fluorometer and NGS Starter Package
核酸およびタンパク質の定量に対応した2チャンネル蛍光測定、NGSアプリケーション向けバンドルも提供
E6150, E5150
Proteinase K Solution
室温保存できる使い安いプロテイナーゼ K 溶液
MC5005, MC5008, MC1111, A5051
