Wizard® SV 96 Genomic DNA Purification System
96-Well, Vacuum-Based DNA Purification From Cells or Tissues
- Suitable for manual or high-throughput applications
- DNA from mouse tails in 45–60 minutes; DNA from cells in 30 minutes
- 20–30μg DNA per prep from 1.2cm mouse tail
Catalog Number:
Size
Catalog Number: A2370
Catalog Number: A2371
Manual or Automated DNA Extraction in 96-Well Plate Format
The Wizard® SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.
With the Wizard® SV Genomic DNA purification system, genomic DNA is purified from cell lysates using 96-well vacuum filtration. Washing the bound DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection trays.
The Wizard® SV Genomic DNA Purification System is designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.
For manual purification, the Wizard® SV Genomic DNA Purification System requires a vacuum pump capable of generating 15–20 inches of Hg or the equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of a vacuum trap (not provided).
Learn more about our custom options for this product at: www.promega.com/custom/
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
Column Wash Solution (CWA) |
A1311 | 1 × 185ml | View Product | |
|
96-Well Deep Well Plate |
A226A | 1 × 1 plate | ||
|
Binding Plate |
A227A | 1 × 1 plate | View Product | |
|
Nuclei Lysis Solution |
A7941 | 1 × 50ml | View Product | |
|
RNase A Solution |
A797C | 1 × 1ml | 4mg/ml | View Product |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product | |
|
EDTA, 0.5M (pH 8.0) |
V423B | 1 × 30ml | View Product | |
|
Wizard® SV Lysis Buffer |
Z3052 | 1 × 50ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
Column Wash Solution (CWA) |
A1318 | 2 × 370ml | View Product | |
|
96-Well Deep Well Plate |
A226A | 4 × 1 plate | ||
|
Binding Plate |
A227A | 4 × 1 plate | View Product | |
|
Nuclei Lysis Solution |
A7941 | 2 × 50ml | View Product | |
|
RNase A Solution |
A797C | 3 × 1ml | 4mg/ml | View Product |
|
Nuclease-Free Water |
P1195 | 2 × 150ml | View Product | |
|
EDTA, 0.5M (pH 8.0) |
V423B | 1 × 30ml | View Product | |
|
Wizard® SV Lysis Buffer |
Z3052 | 3 × 50ml | View Product |
Resources
その他
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