Promega Corporation

GoTaq® Hot Start PolymerasePCR Satisfaction Guaranteed

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase exhibits 5´→3´ exonuclease activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactio...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

GoTaq® Hot Start Polymerase

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GoTaq® Hot Start Polymerase

 Components

  • MgCl2

    A351B1 x 750μl
  • GoTaq® Hot Start Polymerase

    M500A1 x 100u
  • 5X Colorless GoTaq® Flexi Buffer

    M890A1 x 1ml
  • 5X Green GoTaq® Flexi Buffer

    M891A1 x 1ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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100u
- M5001 Please Enquire

GoTaq® Hot Start Polymerase

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GoTaq® Hot Start Polymerase

 Components

  • MgCl2

    A351H3 x 1.2ml
  • GoTaq® Hot Start Polymerase

    M500B1 x 500u
  • 5X Colorless GoTaq® Flexi Buffer

    M890A4 x 1ml
  • 5X Green GoTaq® Flexi Buffer

    M891A4 x 1ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500u
- M5005 Please Enquire

GoTaq® Hot Start Polymerase

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GoTaq® Hot Start Polymerase

 Components

  • MgCl2

    A351H15 x 1.2ml
  • GoTaq® Hot Start Polymerase

    M500B5 x 500u
  • 5X Colorless GoTaq® Flexi Buffer

    M890A20 x 1ml
  • 5X Green GoTaq® Flexi Buffer

    M891A20 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


2,500u
- M5006 Please Enquire

GoTaq® Hot Start Polymerase

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GoTaq® Hot Start Polymerase

 Components

  • MgCl2

    A351H60 x 1.2ml
  • GoTaq® Hot Start Polymerase

    M500B20 x 500u
  • 5X Colorless GoTaq® Flexi Buffer

    M890A80 x 1ml
  • 5X Green GoTaq® Flexi Buffer

    M891A80 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10,000u
- M5008 Please Enquire

GoTaq® Hot Start Green Master Mix

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GoTaq® Hot Start Green Master Mix

 Components

  • GoTaq® Hot Start Green Master Mix, 2X

    M512B2 x 1,250μl
  • Nuclease-Free Water

    P119A2 x 1,250μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100 reactions
- M5122 Please Enquire

GoTaq® Hot Start Green Master Mix

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GoTaq® Hot Start Green Master Mix

 Components

  • GoTaq® Hot Start Green Master Mix, 2X

    M512C1 x 25ml
  • Nuclease-Free Water

    P119C1 x 25ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 reactions
- M5123 Please Enquire

GoTaq® Hot Start Colorless Master Mix

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GoTaq® Hot Start Colorless Master Mix

 Components

  • GoTaq® Hot Start Colorless Master Mix, 2X

    M513B2 x 1,250μl
  • Nuclease-Free Water

    P119A2 x 1,250μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100 reactions
- M5132 Please Enquire

GoTaq® Hot Start Colorless Master Mix

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GoTaq® Hot Start Colorless Master Mix

 Components

  • GoTaq® Hot Start Colorless Master Mix, 2X

    M513C1 x 25ml
  • Nuclease-Free Water

    P119C1 x 25ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 reactions
- M5133 Please Enquire


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Storage Conditions

Store at –30 to –10°C.

For product intended use please see Patents & Disclaimers tab.

GoTaq Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.Figure 1. GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.

Use Restrictions

M5001, M5005, M5006, M5008, M5122, M5123, M5132, M5133 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

M5001, M5005, M5006, M5008, M5122, M5123, M5132, M5133 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

M5001, M5005, M5006, M5008, M5122, M5123, M5132, M5133 Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

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