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GoTaq® Probe 2-Step RT-qPCR SystemPCR Satisfaction Guaranteed

The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system protocol facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components:

  • GoScript™ Reverse Transcription System
  • GoTaq® Probe qPCR Master Mix

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and reverse transcriptase that enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product may be added directly to downst...

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GoTaq® Probe 2-Step RT-qPCR System

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GoTaq® Probe 2-Step RT-qPCR System

 Components

  • MgCl2

    A351B1 x 750μl
  • GoScript™ 5X Reaction Buffer

    A500B1 x 300μl
  • GoScript™ Reverse Transcriptase

    A501B1 x 50μl
  • GoTaq® Probe qPCR Master Mix, 2X

    A610A2 x 1ml
  • Oligo(dT)15 Primer

    C110B1 x 50μg
  • PCR Nucleotide Mix

    C114G1 x 200μl
  • Random Primers

    C118B1 x 50μg
  • CXR Reference Dye

    C541A1 x 100μl
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
  • Nuclease-Free Water

    P119A3 x 1,250μl
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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200 reactions
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Storage Conditions

Store all components between –30°C and –10°C. Protect components from light at all times. For best results, mix thawed solutions gently to minimize aeration and foaming, and keep on ice. For short-term storage and frequent use, the GoTaq® qPCR Master Mix, 2X, may be kept at 2–8°C for up to 3 months if protected from light.

For product intended use please see Patents & Disclaimers tab.

Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.Figure 1. Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.

A series of fivefold serial dilutions of human pancreas cDNA was used to measure GAPDH gene expression using the GoTaq® Probe 2-Step RT-qPCR System and a competing product (Vendor L). The GoTaq® Probe 2-Step RT-qPCR System shows earlier Cq values (>0.2) for all samples. Two microliters of human pancreas RNA was reverse transcribed using the GoScript™ Reverse Transcriptase supplied in the kit followed by qPCR with GoTaq® Probe qPCR Master Mix.

A series of fivefold serial dilutions of human pancreas cDNA was used to measure GAPDH gene expression using the GoTaq® Probe 2-Step RT-qPCR System and a competing product (Vendor L). The GoTaq® Probe 2-Step RT-qPCR System shows earlier Cq values (>0.2) for all samples. Two microliters of human pancreas RNA was reverse transcribed using the GoScript™ Reverse Transcriptase supplied in the kit followed by qPCR with GoTaq® Probe qPCR Master Mix.

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Use Restrictions

A6110 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

A6110 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

A6110 NOTICE TO PURCHASER: DISCLAIMER OF LICENSE
No license is conveyed with the purchase of this product under any of US Pat. Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787 and 6,258,569 and corresponding patents outside the United States or any other patents or patent applications relating to the 5´ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

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