Promega Corporation

GoTaq® Real-Time qPCR and RT-qPCR Systems for Dye-Based DetectionPCR Satisfaction Guaranteed

The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for use in real-time quantitative PCR (qPCR and RT-qPCR). The system contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I. Containing the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad range detection for increased reliability, reproducibility and s...

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  • Prices valid for customers of Promega KK only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

GoTaq® qPCR Master Mix

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GoTaq® qPCR Master Mix

 Components

  • GoTaq® qPCR Master Mix, 2X

    A600A5 x 1ml
  • CXR Reference Dye

    C541A1 x 100μl
  • Nuclease-Free Water

    P119E2 x 13ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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Request to add this product to your Helix »


200 × 50µl reactions
- A6001 Please Enquire

GoTaq® qPCR Master Mix

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GoTaq® qPCR Master Mix

 Components

  • GoTaq® qPCR Master Mix

    A60015 x 200 × 50µl reactions
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 × 50µl reactions
- A6002 Please Enquire

GoTaq® 2-Step RT-qPCR System

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GoTaq® 2-Step RT-qPCR System

 Components

  • MgCl2

    A351B1 x 750μl
  • GoScript™ 5X Reaction Buffer

    A500B1 x 300μl
  • GoScript™ Reverse Transcriptase

    A501B1 x 50μl
  • GoTaq® qPCR Master Mix, 2X

    A600A5 x 1ml
  • Oligo(dT)15 Primer

    C110B1 x 50μg
  • PCR Nucleotide Mix

    C114G1 x 200μl
  • Random Primers

    C118B1 x 50μg
  • CXR Reference Dye

    C541A1 x 100μl
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
  • Nuclease-Free Water

    P119E2 x 13ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50 × 20µl RT reactions + 200 × 50µl qPCR reactions
- A6010 Please Enquire

GoTaq® 1-Step RT-qPCR System

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GoTaq® 1-Step RT-qPCR System

 Components

  • MgCl2

    A351B1 x 750μl
  • GoTaq® qPCR Master Mix, 2X

    A600A5 x 1ml
  • Nuclease-Free Water

    P119E2 x 13ml
  • CXR Reference Dye

    C541B1 x 200μl
  • GoScript™ RT Mix for 1-Step RT-qPCR

    M700A1 x 225μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


200 × 50µl reactions
- A6020 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

CXR Reference Dye

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CXR Reference Dye

 Components

  • CXR Reference Dye

    C541A1 x 100μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μl
- C5411 Please Enquire

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Storage Conditions

Upon arrival, store all components at –30 to –10°C, protected from light. For immediate use, components may be stored at 2–8°C, protected from light, for up to 3 months.

For product intended use please see Patents & Disclaimers tab.

8277TBFigure 1. Performance comparison of GoTaq® qPCR Master Mix and company A's master mix.
GoTaq qPCR Master Mix provides high sensitivity in detecting a single-copy gene.Figure 2. qPCR with single-copy detection.

The GAPDH gene was amplified and detected from tenfold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (slope = 3.2; R2 = 0.995).

The GAPDH gene was amplified and detected from tenfold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (slope = 3.2; R2 = 0.995).

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Use Restrictions

A6001, A6002, A6010, A6020, C5411 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

A6001, A6002, A6010, A6020 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

A6001, A6002, A6010, A6020 NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

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