MethylEdge® Bisulfite Conversion System
Rapid Bisulfite Conversion with Minimal DNA Fragmentation
- High-efficiency DNA conversion from as little as 100pg input DNA
- Rapid protocol saves time compared to other conversion systems
- Robust conversion of DNA with reduced DNA fragmentation
Catalog Number:
Size
Catalog Number: N1301
Bisulfite Conversion for DNA Methylation Workflows
MethylEdge® Bisulfite Conversion System はDNA の断片化を最小限に抑えながら 2時間以内に バイサルファイト変換を行える迅速で効率的な方法です。迅速なプロトコールと完全な変換により下流のアッセイに適した変換DNAを最小限の労力と時間で取得することができます。プロメガの精製技術によりMethylEdge® Bisulfite Conversion System は良質なバイサルファイト変換DNAを取得することができます。
- 高効率な DNA 変換
- DNA 断片化を抑えた頑健な DNA 変換
- 迅速なプロトコール
- 室温保存可能な Ready-to-Use な試薬
このシステムはバイサルファイト変換およびDNA クリーンナップに使用できます。変換DNAの用途として バイサルファイト特異的 PCR (BSP)、バイサルファイトシークエンシング (bis-seq)、バイサルファイト特異的HRMおよび COBRA アッセイなどが挙げられます。
Rapid Conversion, Minimal Fragmentation
Realize the full potential of your samples with the most effective bisulfite conversion system available. The MethylEdge™ Bisulfite Conversion System offers a robust system for rapidly performing bisulfite conversion with minimal DNA fragmentation.
- High-efficiency DNA conversion from as little as 100pg input DNA
- Rapid Protocol saves time compared to other conversion systems
- Robust conversion of DNA with reduced DNA fragmentation
- Ready-to-use reagents stored at room temperature
Degree of DNA Fragmentation After Conversion
DNA Purification and Quantitation
Manual and High-Throughput Chemistries
ReliaPrep™ Genomic DNA Purification Systems offer comprehensive solutions for reliable purification of high-quality gDNA. Kits include manual systems for purification from FFPE samples, blood and tissue and high-throughput gDNA purification from large volume blood samples.
Hands-Free Automated Purification from 1–16 Samples
The Maxwell® RSC Instrument and DNA Purification Kits provide automated DNA extraction from many sample types, including FFPE tissues. The convenient, hands-free extraction method frees up time and provides consistent yields of highly pure gDNA.
Chemistry
The QuantiFluor™ dsDNA System offers sensitive detection of small amounts of double stranded DNA in solution upstream of bisulfite conversion.
Downstream Analysis
| End-Point PCR Systems | Dye-Based Real-Time Systems | Probe-Based Real-Time Systems |
|---|---|---|
|
GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. |
GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad range detection for increased reliability, reproducibility and sensitivity. The system contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum efficiency, and greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. |
The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format. It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). |
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Collection Tubes |
N124A | 1 × 50/pack |
ME Spin Columns |
N125A | 1 × 50/pack |
ME Elution Buffer |
N126A | 1 × 1ml |
ME Desulphonation Buffer |
N127A | 1 × 10ml |
ME Wash Buffer |
N128A | 1 × 6ml |
ME Binding Buffer |
N129A | 1 × 30ml |
ME Conversion Reagent |
N130A | 5 × 1.3ml |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
See Protocol for storage recommendations.
Use of Methylation-Specific PCR (MSP) is protected by U.S. Pat. Nos. 5,786,146, 6,017,704, 6,200,756 and 6,265,171. No license under these patents to use the MSP process is conveyed expressly or by implication to the purchaser by the purchase of this product.
Resources
No related resources available
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