Promega Corporation

Regulated Mammalian Expression System

The Regulated Mammalian Expression System features low basal levels, robust and rapid induction, and downregulation of gene expression in mammalian cells. The Regulated Mammalian Expression System is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumerin-related compounds of a chimeric transactivator protein. Nanomolar concentrations of the antibiotic coumermycin promote homodimerization of a chimeric transactivator that, in turn, binds to lambda operator sequences located upstream of a minimal promoter driving transcription of coding sequences for a protein of interest. The l...

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Regulated Mammalian Expression System

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Regulated Mammalian Expression System

 Components

  • pReg neo Vector

    C942A1 x 20μg
  • pF12A RM Flexi® Vector

    C943A1 x 20μg
  • pF12K RM Flexi® Vector

    C944A1 x 20μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1 system
- C9470 Please Enquire

Coumermycin A1

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Coumermycin A1

 Components

  • Coumermycin A1

    C945A1 x 5mg
5mg
- C9451 Please Enquire

Novobiocin Sodium Salt

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Novobiocin Sodium Salt

 Components

  • Novobiocin Sodium Salt

    C946A1 x 1g
1g
- C9461 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

pReg neo Vector

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pReg neo Vector

 Components

  • pReg neo Vector

    C942A1 x 20μg
20μg
- C9421 Please Enquire

pF12A RM Flexi® Vector

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pF12A RM Flexi® Vector

 Components

  • pF12A RM Flexi® Vector

    C943A1 x 20μg
20μg
- C9431 Please Enquire

pF12K RM Flexi® Vector

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pF12K RM Flexi® Vector

 Components

  • pF12K RM Flexi® Vector

    C944A1 x 20μg
20μg
- C9441 Please Enquire

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GenBank®/EMBL Accession Number

pReg neo Vector Accession Number EF030522; pReg neo Vector sequence text file
pF12A RM Flexi® Vector, EF030520; pF12A RM Flexi® Vector sequence text file.
pF12K RM Flexi® Vector, EF030521; pF12K RM Flexi®Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Representation of the coumerin-regulated system.Figure 1. Representation of the coumerin-regulated system.

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pReg neo Vector.Figure 2. pReg neo Vector.
pF12A RM Flexi Vector.Figure 3. pF12A RM Flexi® Vector.
pF12K RM Flexi Vector.Figure 4. pF12K RM Flexi® Vector.

Use Restrictions

C9470, C9451, C9421, C9431, C9441 For Research Use Only. Not for Use in Diagnostic Procedures.
C9461 For Research Use Only. Not for Use in Therapeutic or Diagnostic Procedures.

Patents - Disclaimers

C9470, C9421, C9431, C9441 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product in their own research and they may transfer derivatives to others for research use provided third parties agree to be bound by the terms of this label license. No commercial use is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, please contact Promega for licensing information. No other use or transfer of this product or derivatives is authorized without the prior express, written consent of Promega. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

C9470, C9431, C9441 European Pat. No. 1685247 and other patents pending.

C9470, C9431, C9441 Patent Pending.

C9470, C9431, C9441 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

C9470, C9421, C9431, C9441 Licensed from National Research Council of Canada.

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