Promega Corporation

HaloTag® Mammalian Protein Purification System

The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent de...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

HaloTag® Mammalian Protein Detection and Purification System

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HaloTag® Mammalian Protein Detection and Purification System

 Components

  • HaloLink™ Resin

    G19131 x 2.5ml
  • HaloTag® TMRDirect™ Ligand

    G299A1 x 30μl
  • Protease Inhibitor Cocktail, 50X

    G652A2 x 1ml
  • HaloTEV Protease

    G660A1 x 1,000u
  • Spin Column

    V995A1 x 25 each
1 each
- G6795 Please Enquire

HaloTag® Mammalian Protein Purification System

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HaloTag® Mammalian Protein Purification System

 Components

  • HaloLink™ Resin

    G19131 x 2.5ml
  • Protease Inhibitor Cocktail, 50X

    G652A2 x 1ml
  • HaloTEV Protease

    G660A1 x 1,000u
  • Spin Column

    V995A1 x 25 each
1 each
- G6790 Please Enquire

HaloTag® Mammalian Protein Detection and Purification System Sample Pack

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HaloTag® Mammalian Protein Detection and Purification System Sample Pack

 Components

  • HaloLink™ Resin

    G191A1 x 0.5ml
  • HaloTag® TMRDirect™ Ligand

    G299B1 x 15μl
  • Protease Inhibitor Cocktail, 50X

    G652A1 x 1ml
  • HaloTEV Protease

    G660C1 x 250u
  • Spin Column

    V995B1 x 10 each
1 each
- G6799 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

HaloTEV Protease

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HaloTEV Protease

 Components

  • HaloTEV Protease

    G660A1 x 1,000u
1,000u
5u/μl G6601 Please Enquire

HaloTEV Protease

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HaloTEV Protease

 Components

  • HaloTEV Protease

    G660B1 x 4,000u
4,000u
5u/μl G6602 Please Enquire

HaloTag® TMRDirect™ Ligand

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HaloTag® TMRDirect™ Ligand

 Components

  • HaloTag® TMRDirect™ Ligand

    G299A1 x 30μl
30μl
0.1mM G2991 Please Enquire

Protease Inhibitor Cocktail, 50X

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Protease Inhibitor Cocktail, 50X

 Components

  • Protease Inhibitor Cocktail, 50X

    G652A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1ml
- G6521 Please Enquire

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Storage Conditions

Store the Spin Columns at room temperature. Store the HaloLink™ Resin at 4°C. Store the HaloTEV Protease, HaloTag® TMRDirect™ Ligand and powdered Protease Inhibitor Cocktail at –30 to –10°C. Reconstituted Protease Inhibitor Cocktail can be stored at 2–10°C  for 12 months.

For product intended use please see Patents & Disclaimers tab.

Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.Figure 1. Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.

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Use Restrictions

G6795, G6790, G6799, G6601, G6602, G2991, G6521 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G6795, G6790, G6799, G2991 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag® ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G6601, G6602 Patent Pending.

G2991 U.S. Pat. No. 7,867,726 and other patents and patents pending.

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