Promega Corporation

HisLink™ Protein Purification Systems

HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. All product formats use the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin). The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. The HisLink™ Protein Purification Resin is useful in all general ...

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  • Prices valid for customers of Promega KK only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

HisLink™ Spin Protein Purification System

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HisLink™ Spin Protein Purification System

 Components

  • Collection Tubes

    A130D1 x 25/pk
  • FastBreak™ Cell Lysis Reagent, 10X

    V857D1 x 5ml
  • HisLink™ Protein Purification Resin

    V882C1 x 5ml
  • HisLink™ Binding/Wash Buffer

    V981A1 x 50ml
  • HisLink™ Elution Buffer

    V982A1 x 10ml
  • Spin Column

    V995A1 x 25 each
  • DNase I (lyophilized)

    Z358B1 x 1 vial
25 reactions
- V1320 Please Enquire

HisLink™ 96 Protein Purification System

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HisLink™ 96 Protein Purification System

 Components

  • HisLink™ 96 Filtration/Collection Plates

    V484A1 x 1 pack
  • Binding/Wash Buffer

    V851C1 x 110ml
  • Elution Buffer

    V852C1 x 25ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
  • HisLink™ Protein Purification Resin

    V882B1 x 25ml
  • DNase I (lyophilized)

    Z358B1 x 1 vial
1 × 96
- V3680 Please Enquire

HisLink™ 96 Protein Purification System

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HisLink™ 96 Protein Purification System

 Components

  • HisLink™ 96 Filtration/Collection Plates

    V484A5 x 1 pack
  • Binding/Wash Buffer

    V851D2 x 280ml
  • Elution Buffer

    V852D1 x 125ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
  • HisLink™ Protein Purifiication Resin

    V882A2 x 50ml
  • DNase I (lyophilized)

    Z358A1 x 1 vial
5 × 96
- V3681 Please Enquire

HisLink™ Protein Purification Resin

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HisLink™ Protein Purification Resin

 Components

  • HisLink™ Protein Purification Resin

    V882C1 x 5ml
5ml
- V8823 Please Enquire

HisLink™ Protein Purification Resin

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HisLink™ Protein Purification Resin

 Components

  • HisLink™ Protein Purifiication Resin

    V882A1 x 50ml
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  • This product can be added to a

  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50ml
- V8821 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
15ml
- V8571 Please Enquire

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
60ml
- V8572 Please Enquire

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857C1 x 100ml
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  • This product can be added to a

  • Helix Cabinet, Refrigerator

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100ml
- V8573 Please Enquire

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Storage Conditions

Store the systems and the resin at 4°C. Plates may be stored at 4°C or room temperature. Store Spin Columns, Collection Tubes and FastBreak™ Cell Lysis Reagent at room temperature. After reconstitution, store the DNase I in aliquots at –20°C.

For product intended use please see Patents & Disclaimers tab.

Overview of polyhistidine- or HQ-tagged protein purification using the HisLink Spin Protein Purification System.Figure 1. Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.
Overview of polyhistidine-tagged protein purification using the HisLink 96 Protein Purification System.Figure 2. Overview of polyhistidine-tagged protein purification using the HisLink™ 96 Protein Purification System.
Vac-Man Laboratory Vacuum Manifold.Figure 3. Vac-Man® Laboratory Vacuum Manifold.

Patents - Disclaimers

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