Promega Corporation

Nuclear Receptor Analysis Luciferase Vectors

See All pGL4 Vectors
Nuclear receptor analysis can be performed with traditional means by using a minimal promoter vector with nuclear receptor response elements upstream. Alternatively, you can use viral elements like the mouse mammary tumor virus long terminal repeat promoter to judge androgen or glucocorticoid responses (e.g., pGL4.36). In many cases, study with these methods requires use of a cell line with the appropriate endogenous nuclear receptors, meaning you may need different cell lines for each nuclear receptor study. A method using the principles of the yeast two-hybrid system was adapted for ...

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pGL4.36[luc2P/MMTV/Hygro] Vector

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pGL4.36[luc2P/MMTV/Hygro] Vector

 Components

  • pGL4.36[luc2P/MMTV/Hygro] Vector

    E136A1 x 20μg
20μg
- E1360 Please Enquire

pFN26A (BIND) hRluc-neo Flexi® Vector

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pFN26A (BIND) hRluc-neo Flexi® Vector

 Components

  • pFN26A (BIND) hRluc-neo Flexi® Vector

    E138A1 x 20μg
20μg
- E1380 Please Enquire

pBIND-ERα Vector

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pBIND-ERα Vector

 Components

  • pBIND-ERα Vector

    E139A1 x 20μg
20μg
- E1390 Please Enquire

pBIND-GR Vector

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pBIND-GR Vector

 Components

  • pBIND-GR Vector

    E158A1 x 20μg
20μg
- E1581 Please Enquire

pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector

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pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector

 Components

  • pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector

    E137A1 x 20μg
20μg
- E1370 Please Enquire

GloResponse™ 9XGAL4UAS-luc2P HEK293 Cell Line

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GloResponse™ 9XGAL4UAS-luc2P HEK293 Cell Line

 Components

  • 9XGAL4UAS-luc2P HEK293 Cell Line

    E853A2 x 1 vial
2 vials
- E8530 Please Enquire


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GenBank®/EMBL Accession Number

pGL4.36[luc2P/MMTV/Hygro] Vector Accession Number FJ773214; pGL4.36[luc2P/MMTV/Hygro] Vector sequence text file.
pFN26A (BIND) hRluc-neo Flexi® Vector Accession Number GQ229578; pFN26A (BIND) hRluc-neo Flexi® Vector sequence text file.
pBIND-ERα Vector Accession Number GQ229579; pBIND-ERα Vector sequence text file.
pBIND-GR Vector Accession Number GQ229580; pBIND-GR sequence text file.
pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector Accession Number GQ229577; pGL4.35[luc2P/9XGAL4UAS/Hygro] sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Representation of the one-hybrid system.Figure 1. Representation of the one-hybrid system.

In this system, the nuclear receptor ligand binding domain (LBD) is fused to the DNA-Binding Domain of the yeast GAL4 transcription factor. The hybrid fusion-protein nuclear receptor is then used to activate the luc2P luciferase reporter under the control of nine repeats of the GAL4 Upstream Activator Sequence (UAS).

In this system, the nuclear receptor ligand binding domain (LBD) is fused to the DNA-Binding Domain of the yeast GAL4 transcription factor. The hybrid fusion-protein nuclear receptor is then used to activate the luc2P luciferase reporter under the control of nine repeats of the GAL4 Upstream Activator Sequence (UAS).

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pGL4.36[luc2P/MMTV/Hygro] Vector.Figure 2. pGL4.36[luc2P/MMTV/Hygro] Vector.
pFN26A (BIND) hRluc-neo Flexi Vector. Figure 3. pFN26A (BIND) hRluc-neo Flexi® Vector.
pBIND-ER-alpha Vector. Figure 4. pBIND-ER-alpha Vector.
pBIND-GR Vector.Figure 5. pBIND-GR Vector.
pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector.Figure 6. pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector.
E2 titration of GloResponse 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-ER-alpha Vector.Figure 7. E2 titration of GloResponse™ 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-ER-alpha Vector.

A total of 10,000 transfected GloResponse™ 9XGAL4UAS-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and fourfold serial dilutions of E2 were added to induce reporter gene expression. After a 24-hour induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Thermo VarioSkan Luminometer. n = 4 for each data point.

Dexamethasone titration of GloResponse 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-GR Vector.Figure 8. Dexamethasone titration of GloResponse™ 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-GR Vector.

A total of 10,000 GloResponse™ 9XGAL4UAS-luc2P HEK293 cells were dispensed into each well of a 384-well plate, and fivefold serial dilutions of dexamethasone were added to induce reporter gene expression. After a 24-hour induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Thermo VarioSkan Luminometer. n = 4 for each data point.

Use Restrictions

E1360, E1380, E1390, E1581, E1370, E8530 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E1360, E1380, E1390, E1581, E1370, E8530 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

E1360, E1370, E8530 U.S. Pat. No. 5,670,356.

E1360, E1380, E1390, E1581, E1370, E8530 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

E1360, E1370 U.S. Pat. No. 7,728,118.

E1380 European Pat. No. 1685247 and other patents pending.

E1380, E8530 Patent Pending.

E1380 U.S. Pat. No. 7,906,282 and European Pat. No. 1341808.

E1360, E1370, E8530 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

E8530 Commercial use of this cell line requires a license from AdVec Inc.

E8530 Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.

E8530 HEK293 cells were obtained under license from AdVec Inc.

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