Promega Corporation

Primer Extension System—AMV Reverse Transcriptase

Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega KK only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Primer Extension System-AMV Reverse Transcriptase

Close Window

Primer Extension System-AMV Reverse Transcriptase


  • 1.2kb Kanamycin Positive Control RNA

    C154A1 x 100ng
  • Loading Dye

    E343B1 x 1ml
  • Primer Extension 2X Buffer

    E349B1 x 500μl
  • Primer Extension Sodium Pyrophosphate

    E350B1 x 75μl
  • ΦX174 DNA/HinfI Dephosphorylated Markers

    E351A1 x 500ng
  • Primer Extension Control Primer

    E355A1 x 50pmol
  • AMV Reverse Transcriptase

    E357B1 x 50u
  • T4 Polynucleotide Kinase

    M410A1 x 100u
  • T4 Polynucleotide Kinase 10X Buffer

    Q561A1 x 100μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

Click here to learn more about the Helix Collection »

Already a Helix Customer?

Request to add this product to your Helix »

40 reactions
- E3030 Please Enquire

Storage Conditions

All components must be stored at –20°C, except for the control RNA, which must be stored at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

E3030 For Research Use Only. Not for Use in Diagnostic Procedures.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.