Promega Corporation

Cell ID™ System

The Cell ID™ System provides the reagents required for successful and simple identification and authentication of human cell lines and detection of intra-species cell line cross-contamination. The Cell ID™ System uses short tandem repeat (STR) analysis of specific loci in the human genome through co-amplification and three-color detection of ten loci (nine STR loci and Amelogenin for gender identification), including D21S11, TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818. All ten loci are amplified simultaneously in a single tube. The Cell ID™ System includes a hot-start Taq...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Cell ID™ System

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Cell ID™ System

 Components

  • K562 DNA High Molecular Weight

    DD208A1 x 3μg
  • Water, Amplification Grade

    DW099A1 x 1,250μl
  • Cell ID™ 10X Primer Pair Mix

    G934A1 x 125μl
  • Cell ID™ 5X Enzyme Mix

    G935A1 x 250μl
  • Cell ID™ Allelic Ladder

    G937A1 x 50μl
  • Internal Lane Standard 600

    DG107A1 x 150μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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50 reactions
- G9500 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

Water, Amplification Grade

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Water, Amplification Grade

 Components

  • Water, Amplification Grade

    DW099A5 x 1,250μl
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  • This product can be added to a

  • Helix Cabinet, Freezer, Refrigerator

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet, a –20°C Helix Freezer or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.


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6,250μl
(5 × 1,250μl)
- DW0991 Please Enquire

Internal Lane Standard 600

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Internal Lane Standard 600

 Components

  • Internal Lane Standard 600

    DG107A1 x 150μl
  • Blue Dextran Loading Solution

    DV435A1 x 1ml
  • Bromophenol Blue Loading Solution

    DV437A1 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Login to request to add this product to your Helix »


150μl
- DG1071 Please Enquire

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Storage Conditions

Store at –20°C. The Primer Pair Mix, Allelic Ladder and Internal Lane Standard are light-sensitive and should be stored protected from light.

For product intended use please see Patents & Disclaimers tab.

Figures

7426MA_600pxFigure 1. Allele ranges for the Cell ID™ System. STR fragments amplified by the Cell ID™ System are labeled with different dyes and are separated by capillary electrophoresis based on size. A size standard is included in each sample to determine the sizes of the Cell ID™-amplified fragments. Fluorescein-labeled loci are shown in dark gray, JOE-labeled loci are shown in gray and TMR-labeled loci are shown in white. The CXR-labeled Internal Lane Standard 600 fragments are represented by black bars.

STR fragments amplified by the Cell ID™ System are labeled with different dyes and are separated by capillary electrophoresis based on size. A size standard is included in each sample to determine the sizes of the Cell ID™-amplified fragments. Fluorescein-labeled loci are shown in dark gray, JOE-labeled loci are shown in gray and TMR-labeled loci are shown in white. The CXR-labeled Internal Lane Standard 600 fragments are represented by black bars.

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Representative data for the Cell ID System.Figure 2. Representative data for the Cell ID™ System.

Genomic DNA from K562 cells (2.0ng) was amplified using the Cell ID™ System. Amplification products were mixed with Internal Lane Standard 600 and analyzed with an Applied Biosystems 3130xl Genetic Analyzer using a 1.5kV, 5-second injection. The results were analyzed using GeneMapper® ID software, version 3.2, and the appropriate panel and bin files.

Genomic DNA from K562 cells (2.0ng) was amplified using the Cell ID™ System. Amplification products were mixed with Internal Lane Standard 600 and analyzed with an Applied Biosystems 3130xl Genetic Analyzer using a 1.5kV, 5-second injection. The results were analyzed using GeneMapper® ID software, version 3.2, and the appropriate panel and bin files.

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Use Restrictions

G9500 For Research Use Only. Not for Use in Diagnostic Procedures.
DW0991 Not For Medical Diagnostic Use.
DG1071 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

U.S. Pat. Nos. 5,843,660 and 6,221,598, Australian Pat. No. 724531, Canadian Pat. No. 2,118,048 and other patents and patents pending.

STR loci are the subject of U.S. Pat. No. RE 37,984, German Pat. No. DE 38 34 636 C2 and other patents issued to the Max-Planck-Gesellschaft zur Förderung der Wissenschaften, e.V., Germany.

This product is sold under licensing arrangements with the USB Corporation. The purchase price of this product includes limited, nontransferable rights under U.S. Patent Application Serial Number 11/171,008 owned by the USB Corporation to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the "Forensic and Genetic Identity Applications Field"). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the USB Corporation, 26111 Miles Road, Cleveland, OH 44128.

This product is sold under licensing arrangements with Stratagene. The purchase price of this product includes limited, nontransferable rights under U.S. Pat. Nos. 5,449,603, 5,605,824, 5,646,019 and 5,773,257 owned by Stratagene to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the "Forensic and Genetic Identity Applications Field"). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the Business Development Department, Stratagene California, 11011 North Torrey Pines Road, La Jolla, CA 92037.

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