Eukaryotic cell-free protein expression systems, such as the TnT® systems, provide a rapid method for protein expression that avoids the solubility problems associated with bacterial expression. Soluble, functional protein can be produced from plasmid DNA in one to four hours. Here we demonstrate the advantages of eukaryotic cell-free expression using ten difficult-to-express proteins and the TnT® T7 Insect Cell Extract and TnT® Quick Coupled Transcription/Translation Systems.
Promega Notes 101, 19–21.