MagneGST™ Pull-Down System Technical Manual

Literature # TM249

The MagneGST™ Pull-Down System provides GSH-linked magnetic particles that allow simple immobilization of GST-fusion bait proteins from bacterial lysates, and an in vitro transcription/translation system for expression of prey proteins. Prey proteins are isolated using MagneGST™ (GSH)Particles containing the appropriate bait protein. The MagneGST™ Pull-Down System protocol can be used for detection of interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TNT® System.