Promega Corporation

Wizard® Plus Minipreps for the Isolation of Binary Plasmids from Agrobacterium...

Wizard® Plus Minipreps for the Isolation of Binary Plasmids from Agrobacterium tumefaciens

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Abstract

Minor modification of the Wizard® Plus Minipreps DNA Purification System procedure allows for the efficient isolation of binary vector DNA from Agrobacterium tumefaciens. Following purification, plasmid DNA can be digested with restriction endonucleases and analyzed by gel electrophoresis. The modified procedure serves as an effective method for screening A. tumefaciens cells for the presence of introduced binary vectors.

Promega Notes 70, 23.

Ross A. Rupert, and Paul J. Bottino

Department of Cell Biology and Molecular Genetics, University of Maryland at College Park
Publication Date: 1999

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