Lumit® IL-2 (Human) Immunoassay
No-Wash Immunoassay for IL-2 Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6020
Catalog Number: W6022
Catalog Number: W6021
Catalog Number: W122A-C
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit® IL-2 Immunoassay quantitatively measures released IL-2 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
- 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
- 500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
- 1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.
How the Lumit® IL-2 Immunoassay Works
Primary antibodies to IL-2 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-2, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-2 levels.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of Lumit® IL-2 Detection
The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.
|
Assay |
IL-2 Human Immunoassay |
|
|
Limit of Detection (LOD) |
11.2pg/ml (3 SD above background) |
|
|
Dynamic Range |
28.2pg/ml – 25ng/ml |
|
|
Minimal Detectable Dose (MDD) |
7.2pg/ml (2 SD above background) |
|
|
Assay Time |
70 minutes |
|
|
Sample Type |
Cell culture supernatants |
|
SD=Standard Deviation
Detect IL-2 Release as a Marker of T Cell Activation
Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Lumit® IL-2 (Human) Immunoassay reagents were added directly to treated cell wells to detect IL-2 release from the T cells.
Lumit® IL-2 Immunoassay is Scalable for High-Throughput Applications
Human PBMCs were plated in 96-well plates (left) or 384-well plates (right) at 40,000 cells/well or 10,000 cells/well, respectively, and treated with a cell stimulation cocktail to induce IL-2 release. The Lumit® IL-2 Immunoassay reagents were added directly to the cell culture wells. The calculated EC50 values are similar in the 96-well plate and 384-well plate formats.
Explore Additional Cytokine Detection Assays
We offer a growing portfolio of assays to detect cytokines in cell culture samples.
Can’t find your target in the list?
Please enquire about early access to new assays in development.
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Human IL-2 Standard |
W122A | 1 × 25μl | 10μg/ml |
Anti-hIL-2 mAb-SmBiT, 250X |
W123A | 1 × 60μl | |
Anti-hIL-2 mAb-LgBiT, 250X |
W124A | 1 × 60μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Human IL-2 Standard |
W122A | 1 × 25μl | 10μg/ml |
Anti-hIL-2 mAb-SmBiT, 250X |
W123A | 5 × 60μl | |
Anti-hIL-2 mAb-LgBiT, 250X |
W124A | 5 × 60μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Human IL-2 Standard |
W122A | 1 × 25μl | 10μg/ml |
Anti-hIL-2 mAb-SmBiT, 250X |
W123B | 1 × 600μl | |
Anti-hIL-2 mAb-LgBiT, 250X |
W124B | 1 × 600μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Human IL-2 Standard |
W122A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
- Rapid and Sensitive Determination of Cytokine Release from Cells Without Sample Transfer
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
Related Products
Similar Products
Lumit® IL-4 (Human) Immunoassay
Quantitatively measures released IL-4 in cell culture samples using a simple, no-wash protocol.
W6060, W6062, W6061, W125A-C
Lumit® IL-6 (Human) Immunoassay
Quantitatively measures released IL-6 in cell culture samples using a simple, no-wash protocol.
W6030, W6032, W6031, W128A-C
Lumit® IFN-γ (Human) Immunoassay
Quantitatively measures released IFN-γ in cell culture samples using a simple, no-wash protocol.
W6040, W6042, W6041, W134A-C
Lumit® TNF-α (Human) Immunoassay
Quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol.
W6050, W6052, W6051, W137A-C
Frequently Used With
T Cell Activation Bioassay (NFAT/IL-2)
Choose NFAT or IL-2 kit to assess activity of biologics designed to bind and activate T cells via engagement of TCR/CD3.
J1621, J1625, J1601, J1651, J1655, J1631
CellTox™ Green Cytotoxicity Assay
Measures changes in membrane integrity. Kinetically monitors cytotoxicity up to 72 hours with multiplex capability.
G8741, G8742, G8743, G8731
Lumit® Immunoassay Labeling Kit and Detection Reagents
Antibody labeling kit and detection reagents to help develop Lumit® Immunoassays.
VB2500, VB2010, VB2020, VB2030, VB4050, VB4060
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
