pF4 CMV Flexi® Vectors
Untagged Proteins Expressed in Mammalian Cells Without a Selectable Marker
- Expresses high-levels of protein from cloned insert with CMV promoter
- Transfer your cloned insert into other Flexi® Vectors for additional applications (e.g., bacterial or cell-free protein expression) as needed
- Adapts to high-throughput formats for large screening projects, enhancing productivity
Catalog Number:
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Size
Catalog Number: C8481
Catalog Number: C8491
The pF4A and pF4K CMV Flexi® Vectors are designed specifically for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker. All inserts may be confirmed by cell-free expression with the TnT® T7 Quick System (Cat.# L1170). There are no licensing fees or complicated transfer restrictions for these vectors. Clone your ORFs and conveniently transfer the inserts to a variety of Flexi® Vectors designed for multiple applications.
About the Flexi® Vector System
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
Ready-to-Use HaloTag® Flexi® Vector Clones
For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.kasuza.or.jp.kop/dsearch-e
Sequence Information
pF4A CMV Flexi® Vector GenBank® Accession Number AY753580 and vector sequence text file.
pF4K CMV Flexi® Vector GenBank® Accession Number AY753581 and vector sequence text file.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pF4A CMV Flexi® Vector |
C848A | 1 × 20μg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pF4K CMV Flexi® Vector |
C849A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
Resources
Software and Firmware
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Frequently Used With
HaloLink™ Resin
HaloTag® ligand bound to Sepharose beads that specifically and rapidly binds HaloTag® fusion proteins.
G1912, G1913, G1914, G1915
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A 61kDa purified HaloTag®-GST fusion protein supplied at a concentration of 3.0mg/ml. Use to estimate the expression level of your HaloTag® fusion protein.
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BL21(DE3)pLysS Competent Cells
Ready-to-transform E. coli cells with IPTG-inducible protein expression from the T7 promoter.
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TnT® Quick Coupled Transcription/Translation System
Coupled transcription/translation system that produces significant quantities of protein for applications including GST pull-downs and gel shift assays in 60–90 minutes.
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TnT® SP6 High-Yield Wheat Germ Protein Expression System
A single-tube, coupled transcription/translation system that expresses protein in concentrations of 10–100μg/ml in about two hours. Protein is suitable for applications including high-throughput analysis.
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HaloTag® Fluorescent Ligands
For protein labeling and detection. Each includes a chloroalkane group for HaloTag® protein attachment.
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HaloTag® Ligand Building Blocks
Enables any reporter, protein or nucleic acid with specified functional groups to be used with HaloTag® protein.
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Anti-HaloTag® pAb
Polyclonal rabbit antibody that specifically detects HaloTag® fusion proteins.
G9281
Anti-HaloTag® Monoclonal Antibody
Monoclonal antibody raised in mice against the HaloTag® protein for detecting HaloTag® fusion proteins in Western blotting.
G9211
