AgeI
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
Catalog Number:
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Size
Catalog Number: R7251
Cut Site:
A▼CCGG T
T GGCC▲A
Usage Note: Ends generated with AgeI can be directly ligated to ends generated using AccIII, NgoMIV or XmaI. The AgeI recognition site is not regenerated in the ligation product.
Incubation Conditions: Buffer K. 37°C.
Source: Agrobacterium gelatinovorum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol, 0.5mg/ml BSA.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | K | MULTI-CORE™ |
|---|---|---|---|---|---|
| 25–50% | 25–50% | 25–50% | 50–75% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 13 | 5 | 0 | 0 | 0 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer K 10X Buffer |
R010A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
AgeI |
R725A | 1 × 100u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
