DpnI

Cut Site:

G^meA^▼TC
CT_▲^meAG

Usage Note: DpnI requires N⁶-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.

Incubation Conditions: Buffer B. 37°C.

Source: Diplococcus pneumoniae.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: