NdeI

Catalog Number:

Choose a concentration

10u/µl

Size

500u

Catalog Number: R6801

Cut Site:

CA^▼TA TG
GT AT_▲AC

Notes:

Star activity may be observed with glycerol concentrations >10%.
For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Neisseria denitrificans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
<10%	<10%	25–50%	100%	25–50%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
7	2	0	1	3	1

Protocols

Catalog Number:

Item	Part #	Size	Concentration	Available Separately
Buffer D 10X Buffer	R004A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
NdeI	R680A	1 × 500u
MULTI-CORE™ 10X Buffer	R999A	1 × 0.25ml		View Product