Glycerol-Glo™ Assay
Simple, Sensitive Glycerol Detection Assay for a Variety of Biological Samples
- Works with lysed cells, tissues, medium and serum samples
- Perform directly in-well
- Linear glycerol detection up to 80µM
Catalog Number:
Size
Catalog Number: J3150
Catalog Number: J3151
Rapid and Sensitive Glycerol Detection in Biological Samples
The Glycerol-Glo™ Assay is a bioluminescent assay for rapid and sensitive measurement of glycerol in a variety of biological samples, including cells grown in monolayer or 3D structures, cell culture medium, tissues and serum samples. Glycerol is often measured as the product of lipolysis, where it is released from triglycerides. Glycerol is also a substrate or product of many other enzymatic or metabolic processes that can be studied with the glycerol assay. Any processes that result in changes in glycerol concentration, both extracellular and intracellular, can be studied with the Glycerol-Glo™ Assay.
How the Assay Works
The Glycerol-Glo™ Assay measures glycerol in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. Glycerol kinase and glycerol-3-phosphate dehydrogenase are used to generate NADH.
In the presence of NADH, Reductase enzymatically reduces a proluciferin Reductase Substrate to luciferin. Luciferin is detected in a luciferase reaction using Ultra-Glo™ Luciferase and ATP and the amount of light produced is proportional to the amount of glycerol in the sample.
Simple, Flexible Sample Preparation
Glycerol Release from Adipocytes
The Glycerol-Glo™ Assay can be used with cultured cells, including 3D cell culture models, homogenized tissue samples, cell culture medium samples and serum or plasma samples.
No organic extraction is required. The Glycerol Lysis Reagent effectively extracts glycerol from your sample in a single reagent addition without extreme heat, centrifugation steps or multi-step organic extraction protocols.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Reductase Substrate |
G885A | 1 × 55μl | |
Glycerol Lysis Solution |
J315A | 1 × 10ml | |
Kinetic Enhancer |
J316A | 1 × 50μl | |
Glycerol Detection Solution |
J317A | 1 × 5ml | |
Glycerol Standard |
J324A | 1 × 500μl | 20mM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Reductase Substrate |
G885B | 2 × 275μl | |
Glycerol Lysis Solution |
J315B | 1 × 100ml | |
Kinetic Enhancer |
J316B | 1 × 500μl | |
Glycerol Detection Solution |
J317B | 1 × 50ml | |
Glycerol Standard |
J324A | 1 × 500μl | 20mM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
Resources
Posters
Other Resources
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