PowerSeq® Whole Mito System
Streamlined Workflow for Complete Mitochondrial Genome MPS Analysis
- Massively parallel sequencing of whole mitochondrial genome in two amplification reactions
- More efficient workflow greatly reduces number of steps and time required for library preparation
- Improved results with compromised samples—generates 161 small amplicons
Catalog Number:
Size
Catalog Number: PS1600
Streamlined Workflow for mtDNA Analysis
Massively parallel sequencing (MPS) allows laboratories access to mitochondrial DNA (mtDNA) analysis using a more sensitive and high-throughput workflow compared to traditional sequencing methods. Increased mixture deconvolution and heteroplasmy resolution are achieved by deep sequencing coverage and digital read counts. Additionally, the use of small amplicons to sequence the mitochondrial control region improves sequencing results from degraded samples.
The PowerSeq® Whole Mito System generates 161 small amplicons covering the entire region of the mitochondrial genome. The targeted regions for amplification are designed for an average size of 167bp to ensure optimal results from degraded samples. The system contains reagents for library preparation and a streamlined workflow that greatly reduces the number of steps and time required to produce libraries ready for sequencing.
Sequencing data can be analyzed using GeneMarker HTS Software (SoftGenetics, LLC).
PowerSeq® Whole Mito System workflow.
Percent major variant, minimum and average coverage across the whole mitochondrial genome. Varying input amounts of positive control 2800M DNA were amplified and sequenced using PowerSeq® Whole Mito System and MiSeq® sequencing reagents and analyzed using GeneMarker® HTS software, version 2.5. Each concentration was sequenced in duplicate, and 22 libraries were pooled in a single run.
PowerSeq® Whole Mito System Components
The PowerSeq® Whole Mito System is comprised of two kits, the PowerSeq® Whole Mito Amp and Prep Kit (Cat.# PS1650) and the PowerSeq® Whole Mito Purification Kit (Cat.# PS2010).
PowerSeq® Whole Mito Amp and Prep Kit (Cat.# PS1650)
Cat.# PS1650 provides sufficient reagents to perform 100 amplification reactions.
| Item | Part# | Size | Available Separately |
| Pre-Amplification Box | |||
| PowerSeq® Whole Mito 5X Master Mix | DP407A | 1 × 500µl | |
| PowerSeq® Whole Mito 10X Primer Mix 1 | DK482A | 1 × 250µl | |
| PowerSeq® Whole Mito 10X Primer Mix 2 | DK483A | 1 × 250µl | |
| Water, Amplification Grade | DW099A | 2 × 1,250µl | View Product |
| 2800M Control DNA, 10ng/µl | DD710A | 1 × 25µl | View Product |
| Post-Amplification Box | |||
| Library Prep Reagent | PS210A | 1 × 400µl | |
| Library Prep Buffer | PS220A | 6 × 500µl | |
PowerSeq® Whole Mito Purification Kit (Cat.# PS2010)
Cat.# PS2010 provides sufficient reagents to perform 100 purifications.
| Item | Part# | Size | Available Separately |
| ProNex® Size-Selective Chemistry |
NG103C | 1 × 25ml | |
| ProNex® Binding Buffer | NG102A | 1 × 4ml | |
| Wash Buffer |
NG105C | 1 × 40ml | |
| Elution Buffer |
NG116C | 1 × 15ml | |
| Nuclease-Free Water | P119L | 1 × 7ml | View Product |
| Proteinase K (PK) Solution | MC500D | 1 × 1ml | View Product |
Protocols
Complete Protocol
Specifications
Catalog Number:
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Resources
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