Luciferase Assay System
Rapid Detection of Firefly Luciferase Activity
- Linear over eight or more orders of magnitude of enzyme concentration
- Sensitive to 10–20 moles of luciferase
- Cell lysis, sample prep and assay complete in as little as 5 minutes
Catalog Number:
Size
Add Reporter Lysis Buffer
Catalog Number: E1500
Catalog Number: E4030
Catalog Number: E1501
Catalog Number: E4530
Catalog Number: E4550
Simple Non-Homogenous Assay to Quantify Firefly Luciferase Activity
The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10–20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.
The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so the assay is compatible with measurement of luciferase using a single-tube luminometer or a multiwell plate luminometer with an auto-injector. A luminometer is preferred, but not required as the assay is adaptable to scintillation counters.
Cell Lysis Reagents used with the Luciferase Assay System
The Luciferase Assay System is a nonhomogeneous assay; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) can be used with the Luciferase Assay System for reporter quantitation in mammalian cells. Reporter Lysis Buffer enables luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. The Luciferase Assay System also can be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications.
In some kits the lysis buffer is included, and in others it must be purchased separately (see the Specifications section of this page to view assay components).
References
- Wood, K.V. (1991) In: Bioluminescence & Chemiluminescence: Current Status, eds. P. Stanley and L. Kricka, John Wiley & Sons, Ltd., Chichester, 543.
- Seliger, H.H. and McElroy, W.D. (1960) Arch. Biochem. Biophys. 88, 136.
- Wood, K.V. et al. (1984) Biochem. Biophys. Res. Comm. 124, 592–6.
- de Wet, J.R. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7870–3.
- Alam, J. and Cook, J.L. (1990) Anal. Biochem. 188, 245–54.
- Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.
- Jones, D.P. et al. (1995) Promega Notes 54, 20–5.
Check out these newer, homogenous firefly luciferase assay options!!
ONE-Glo™ EX Luciferase Assay System
- Moderate signal.
- Moderate half-life (2 hours).
- Ideal for high- or ultrahigh-throughput processing.
Steady-Glo® Luciferase Assay System
- Reduced signal.
- Maximum signal stability (5 hours).
- Ideal for high-throughput applications when extended luminescence is required.
Bright-Glo® Luciferase Assay System
- Brightest signal.
- Short half-life (30 minutes).
- Ideal for high-throughput applications when high sensitivity is required.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Luciferase Assay Substrate |
E151A | 1 × 1 vial | |
|
Luciferase Assay Buffer |
E152A | 1 × 10ml | |
|
Cell Culture Lysis 5X Reagent |
E153A | 1 × 30ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Luciferase Assay Substrate |
E151A | 1 × 1 vial | |
|
Luciferase Assay Buffer |
E152A | 1 × 10ml | |
|
Reporter Lysis 5X Buffer |
E397A | 1 × 30ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Luciferase Assay Substrate |
E151A | 10 × 1 vial |
|
Luciferase Assay Buffer |
E152A | 10 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Luciferase Assay Substrate |
E151A | 10 × 1 vial | |
|
Luciferase Assay Buffer |
E152A | 10 × 10ml | |
|
Reporter Lysis 5X Buffer |
E397A | 10 × 30ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Luciferase Assay Substrate |
E151C | 1 × 1 vial |
|
Luciferase Assay Buffer |
E152B | 1 × 105ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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Frequently Used With
Glo Lysis Buffer
Promotes rapid lysis (5 minutes) of cultured mammalian cells without scraping or freeze-thaw cycles.
E2661
GloMax® Explorer System
Easy-to-use, flexible multimode detection system.
GM3500, GM3510
GloMax® Navigator System
Dedicated microplate luminometer.
GM2000, GM2010
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
