FuGENE® 4K Transfection Reagent
Most Effective Broad-Spectrum DNA Transfection Reagent
- High-efficiency transfection in challenging and routine cell lines
- Gentle reagent minimizes impact on cell viability
- Ideal for protein and virus production
Catalog Number:
Size
Catalog Number: E5911
Catalog Number: E5912
A Best-in-Class DNA Transfection Reagent for Your Research
FuGENE® 4K is the newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into challenging and routine mammalian cell lines.
Improving on previous generations of FuGENE® technology, FuGENE® 4K provides efficient delivery of DNA into a wide range of cell lines while providing the gentle, low-toxicity transfection you’ve come to expect from FuGENE® reagents. Obtain higher transfection efficiencies than leading competitors’ reagents while minimizing impacts on cell health to get the most out of your transfection-based experiments.
Animal-free reagents and strong performance in HEK293 and CHO cells make FuGENE® 4K ideal for protein and viral production. A quick and easy protocol helps you obtain superior results with minimal optimization, freeing valuable lab time.
Unbeatable Transfection Efficiency with Minimal Effect on Cell Health
FuGENE® 4K reagent delivers improved DNA transfection performance while minimizing impact on cell health. A375, CHO-K1, H1299 and HCT116 cell lines were transfected with a GFP expression construct using FuGENE® 4K or Lipofectamine™ 3000 (ThermoFisher Scientific) with varying reagent:DNA ratios. After 48 hours, cells in a clear-bottom plate were measured for total GFP fluorescence. Cell viability was measured on a duplicate plate using the CellTiter-Glo® Luminescent Cell Viability Assay.
Work with the Best Mammalian Cell Line for Your Research
FuGENE® 4K reagent outperforms Lipofectamine™ 3000 across many cell lines. A variety of cell lines were seeded and subsequently transfected with GFP-expressing plasmid using FuGENE® 4K or Lipofectamine™ 3000 transfection reagent. Transfection efficiency was determined using flow cytometry to count cells expressing GFP. Cell viability was determined using propidium iodide dye.
We needed to try a different plasmid transfection reagent for N2A cells (mouse). Our first try with FuGENE 4K worked beautifully and was easy to work with. Since it is a single reagent and everything is mixed in one tube, I preferred testing FuGENE 4K over other options from different vendors. This will be our go-to transfection reagent moving forward.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
FuGENE® 4K Transfection Reagent |
E591A | 1 × 1ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FuGENE is a registered trademark of Fugent, L.L.C., USA.
FuGENE® 4K is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.
What's in the box?
| Item | Part # | Size |
|---|---|---|
FuGENE® 4K Transfection Reagent |
E591A | 5 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FuGENE is a registered trademark of Fugent, L.L.C., USA.
FuGENE® 4K is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.
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