GoTaq® G2 Hot Start Master Mixes
Room-Temperature PCR Setup with High-Performance Master Mix
- All-in-one master mix; just add template and primers
- Eliminate nonspecific amplification with a hot start enzyme
- Use the green master mix for direct-to-gel analysis, and the colorless master mix when post-amplification fluorescence or absorbance analysis is required
Catalog Number:
Choose a master mix
Size
Catalog Number: M7422
Catalog Number: M7423
Catalog Number: M7432
Catalog Number: M7433
Convenient, Room-Temperature Setup and Higher Performance GoTaq® G2 Enzyme
GoTaq® G2 Hot Start Polymerase is bound to a proprietary antibody that blocks activity at temperatures below 70°C for convenient, room-temperature reaction setup. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR following room-temperature reaction assembly. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase also exhibits 5´→3´ exonuclease activity.
The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template, and go!
The GoTaq® G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using the GoTaq® G2 Hot Start Green (G) and Colorless (C) Master Mix and the leading competitors (L1, L2, Q+ and T) according to manufacturers’ protocols.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
GoTaq® G2 Hot Start Green Master Mix |
M742A | 2 × 1.25ml | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
GoTaq® G2 Hot Start Green Master Mix |
M742B | 1 × 25ml | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
GoTaq® G2 Hot Start Colorless Master Mix |
M743A | 2 × 1.25ml | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
GoTaq® G2 Hot Start Colorless Master Mix |
M743B | 1 × 25ml | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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