QuantiFluor® ssDNA System
Sensitive Quantitation of Single-Stranded DNA
- More sensitive than NanoDrop for low-concentration samples
- Requires less DNA than spectrophotometry
Catalog Number:
Size
Catalog Number: E3190
Simple, Accurate DNA Quantitation
The QuantiFluor® ssDNA System contains a fluorescent dye that enables sensitive quantitation of small amounts of single-stranded (ssDNA) in solution. Detecting and quantitating ssDNA is useful many molecular biology research applications, including studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analysis of first-strand cDNAs, and quantitating bisulfate-treated DNA to study DNA methylation.
The QuantiFluor® ssDNA System requires less DNA (200pg) than other methods (~1 µg), so you save more of your sample for downstream assays.
Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems
For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.
The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent.
| Sample* | Assay | |
|---|---|---|
|
QuantiFluor® ssDNA System
|
0.2–400ng/µl
|
0.2–400ng
|
|
Quant-iT™ OliGreen
|
0.2–400ng/µl
|
0.2–400ng
|
|
Qubit® ssDNA
|
1–200ng/µl
|
1–200ng
|
|
NanoDrop® 2000 Spectrophotometer
|
2–12,000ng/µl
|
2–12,000ng
|
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
ssDNA Quantitation Sensitivity and Assay Linearity
The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.
Easy Setup on Microplate or Single-Tube Fluorometers
The QuantiFluor® ssDNA System includes all the necessary reagents to quickly set up and quantitate single-stranded DNA, and is easy to set up on microplate or single-tube fluorometers. The System is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. Compatible Promega fluorometers include:
How Do Fluorescent Dyes Compare to Other Quantitation Methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
20X TE Buffer (pH 7.5) |
E260A | 1 × 25ml | View Product |
QuantiFluor® ssDNA Dye, 200X |
E284A | 1 × 1ml | |
ssDNA Standard |
E288A | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
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