NanoPrism™ Luciferase Technology
Real-time dual-color detection with a single substrate
Combining NanoPrism™ luciferase with NanoLuc® luciferase technology enables dual-color detection of two biological processes simultaneously through spectrally distinct bioluminescence signals. Join our Early Access Waitlist to be among the first to know when it launches.
What is NanoPrism™ luciferase technology?
Complex biological systems require monitoring multiple processes to understand true cause-and-effect relationships and validate experimental results. Traditional single-readout assays only tell part of the story. NanoPrism™ technology adds a second bioluminescent readout to your existing NanoLuc®/NanoBiT® assays, giving you all the benefits of live-cell bioluminescence for both signals in a single sample.
Key capabilities:
- Dual-signal detection with a single substrate addition
- Seamless integration with existing NanoLuc® or NanoBiT® workflows
- Live-cell compatible for real-time monitoring
- Sensitive to endogenous expression levels
Based on methodology published in ACS Chemical Biology
How does NanoPrism™ luciferase technology work?
NanoPrism™ technology was engineered by incorporating circularly permuted NanoLuc (PrismaLuc) or LgBiT (PmLgBiT) into HaloTag. PrismaLuc™/PrismaBiT™ luciferases generate a spectrally distinct bioluminescent signal through energy transfer to a covalently bound fluorophore (e.g., Janelia Fluor® 549 HaloTag® ligand; JF549), enabling quantitative measurement of two biological events when combined with NanoLuc®/NanoBiT® luciferases in the same sample. This dual-color capability works on standard plate readers and bioluminescence imagers using filtered luminescence detection.
Applications of NanoPrism™ technology
Ideal for researchers studying:
- Targeted protein degradation
- Biased signaling
- Protein-protein interactions
- Multi-cell type dynamics
- And more!
Upgrade to Dual-Color Assays with NanoPrism™ Technology
Ready to transform your single-readout assays into powerful dual-color experiments by adding NanoPrism™ technology's bioluminescent signal? Sign up for priority notification when NanoPrism™ technology becomes available.