PIK3R1 Expression Vector

NV4031_PIK3R1 Expression Vector- 20ug
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Regulatory Vector for NanoBRET® Target Engagement

  • Transfection-ready DNA to express full-length PIK3R1 regulatory protein
  • Use with select kinase-NanoLuc® fusion vectors and NanoBRET® TE Intracellular Kinase Assay

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Catalog number selected: NV4031

$ 585.00
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PIK3R1 Expression Vector
3 × 20μg
$ 585.00
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Express Regulatory Proteins in Mammalian Cells

The PIK3R1 Expression Vector contains the coding region of the human PIK3R1 regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The expression vector and kinase-NanoLuc® fusion vector are cotransfected into cells prior to analysis using the appropriate NanoBRET® Target Engagement Intracellular Kinase Assay. These regulatory protein expression vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.

For details on kinase-NanoLuc® Fusion Vectors that require co-expression with a regulatory protein expression vector, see the NanoBRET® Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.

Vector Details:

  • Gene name: PIK3R1
  • Sequence
  • Recommended Assay: NanoBRET® TE Intracellular Kinase Assay, Adherent format

NanoBRET® Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET® TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET® Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET® signal for estimating intracellular affinity against the target protein. The NanoBRET® Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET® Tracer assay is correct for each kinase fusion vector.

Specifications

What's in the box?

Item Part # Size Concentration

PIK3R1 Expression Vector

NV403A 3 × 20μg 1μg/μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Licensed from Kazusa Genome Technologies.

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