すぐに使える CRISPR ノックインレポーター細胞

標的タンパク質に生物発光タグを融合させるためにCRISPR によりノックインしたゲノム編集細胞株は、内在性タンパク質の存在量の変化を調べるための便利で定量可能な方法を提供します。これらの細胞株を用いた生細胞アッセイにより、タンパク質動態のリアルタイムモニタリングが可能になります。

プロメガは、 HiBiT, NanoLuc®, および HaloTag® CRISPR ノックイン細胞のプールとクローンのコレクションを拡充し続け、NおよびC末端融合タンパク質として多くの人気のある薬剤ターゲットを取り揃えています。ノックインは一般的な主力細胞株で行われ、多くの場合、研究されている生物学に最も関連のある細胞バックグラウンドを有しています。私たち専門スタッフに細胞改変を任せいただければ、研究目標に集中していただけます。

Learn more about Knock-In Tagging

細胞株プールとクローンについて

構築済みおよび開発中のCRISPRノックイン細胞株を多数ご用意しています。以下の表を検索して、目的のターゲットまたは細胞バックグラウンドをご選択ください。開発中の細胞株は（*）で示されています。

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Target	Tag	Terminus	Background	Clone/Pool
ADAR1(p150)	HiBiT	N	A549	C
AKT1	HiBiT	C	PC-3	C
APEX	HiBiT	C	HEK293 (LgBiT)	P
AR	HiBiT	C	22Rv1	C
AR	HiBiT	C	LNCaP clone FGC	C
AR(V7)	HiBiT	C	22Rv1	C
ARAF	HiBiT	N	A549	C
ARID1A	HiBiT	N	ES-2	P
ARID1A	HiBiT	C	ES-2	P
ARID1B	HiBiT	N	TOV-21G	P
ARID1B	HiBiT	C	TOV-21G	P
AURKA	HiBiT	C	MOLT-4	P
AURKA	HiBiT	C	PC-3	P
BCL10	HiBiT	C	NCI-H1299	C
BCL11A	HiBiT	C	HEK293 (LgBiT)	C
BCL11A	HiBiT	N	HEK293 (LgBiT)	C
BCL2L1	HiBiT	N	A549	C
BPTF	HiBiT	N	HEK293	P
BPTF	HiBiT	C	HEK293	P
BRAF	HiBiT	N	A549	C/P
BRAF(V600E)	HiBiT	N	A375	C
BRD2	HiBiT	N	HEK293 (LgBiT)	C
BRD3	HiBiT	N	HEK293 (LgBiT)	C
BRD4	HiBiT	N	A549	C
BRD4	HiBiT	N	HEK293	C
BRD4	NanoLuc	N	HEK293	C
BRD4	HiBiT	N	HEK293 (LgBiT)	C
BRD4	HiBiT-HaloTag	N	HEK293 (LgBiT)	C
BRD4	HiBiT	N	PC-3	P
BRD4 (CRBN KO)	HiBiT	N	HEK293 (LgBiT)	C
BRD4 (VHL KO)	HiBiT	N	HEK293 (LgBiT)	C
BRD7	HiBiT	C	HEK293	P
BRD7	HiBiT	C	HEK293 (LgBiT)	C
BRD7	HiBiT	N	HEK293 (LgBiT)	C/P
BRD9	HiBiT	C	HEK293	P
BRD9	HiBiT	C	HEK293 (LgBiT)	C
BRD9	HiBiT	N	HEK293 (LgBiT)	C/P
BRPF1	HiBiT	N	HEK293	P
BRPF1	HiBiT	C	HEK293	P
BTK	HiBiT	C	K562	C
BTK	HiBiT	C	Ramos	C
CBLB	HiBiT	C	Jurkat	C/P
CCNE1	HiBiT	C	HEK293	C
CCNE1	HiBiT	C	NIH:OVCAR-3	C
CCNK	HiBiT	N	A549	C
CDK1	HiBiT	C	HEK293	C
CDK12	HiBiT	C	A549	C
CDK2	NanoLuc	C	HEK293	C
CDK2	HiBiT	C	HEK293	C
CDK4	HiBiT	C	HEK293	P
CDK4	HiBiT	N	K562	P
CDK4	NanoLuc	N	K562	C
CDK4	NanoLuc	N	T-47D	C
CDK6	HiBiT	C	HEK293	C
CDK6	HiBiT	C	K562	P
CDK6	NanoLuc	N	K562	C
CDK7	HiBiT	N	HEK293	C
CDK9	HiBiT	N	HEK293	C
CDK9	HiBiT	C	HEK293	P
CREBBP	HiBiT	C	NCI-H929	P
CREBBP	HiBiT	C	HEK293	P
CREBBP	HiBiT	N	HEK293	P
CREBBP	HiBiT	C	U-2 OS	C/P
CSNK1A1	HiBiT	C	HEK293 (LgBiT)	C/P
CTNNB1	HiBiT	C	DLD-1	C
CTNNB1	HiBiT	N	HEK293 (LgBiT)	C
CTNNB1	HiBiT-HaloTag	C	HEK293 (LgBiT)	C
CYC1	HiBiT	C	BT-549	C
DDX3X	HiBiT	N	HEK293 (LgBiT)	C
deltaN-TP63	HiBiT	N	BxPC-3	C
DGKA (alpha)	HiBiT	N	Jurkat	C
DGKB (beta)	HiBiT	N	A204	C
DGKZ (zeta)	HiBiT	N	Jurkat	C
DPP9	HiBiT	C	HEK293 (LgBiT)	C
EGFR	HiBiT	C	A549 (LgBiT)	C
EGFR	HiBiT	N	HeLa	C
EP300	HiBiT	C	NCI-H929	P
EP300	HiBiT	C	HEK293	P
EP300	HiBiT	N	HEK293	P
EP300	HiBiT	C	U-2 OS	C/P
ERBB3 (HER3)	NanoLuc	C	AU565	C
ESR1 (ER)	HiBiT	C	MCF7	C
EZH2	HiBiT	C	HEK293	C
FANCM	HiBiT	C	HEK293 (LgBiT)	P
FANCM	HiBiT	N	HEK293 (LgBiT)	P
FANCM	HiBiT	N	U-2 OS	C/P
FANCM	HiBiT	C	U-2 OS	C/P
FGFR1	HiBiT	C	A549	C
FGFR1	HiBiT	C	NCI-H1299	C
FOXA1	HiBiT	N	22Rv1	C
GAPDH	HiBiT	C	HEK293	C/P
GSPT1	HiBiT	N	HEK293 (LgBiT)	C/P
HDAC6	HiBiT	C	HeLa (LgBiT)	C
HMOX1	HiBiT	N	A549	C
HRI (EIF2AK1)	HiBiT	C	HEK293	C
HRI (EIF2AK1)	HiBiT	C	K562	C
IKZF1 (Ikaros)	HiBiT	C	Jurkat (LgBiT)	C/P
IKZF2 (Helios)	HiBiT	C	Jurkat (LgBiT)	P
IKZF2 (Helios)	HiBiT	N	Jurkat (LgBiT)	C/P
IKZF3 (Aiolos)	HiBiT	N	MM.1S	C/P
IL17RA	HiBiT	C	HeLa	P
IL2 (CD38 KO)	NanoLuc	C	Jurkat	C
IL4R	HiBiT	C	THP-1	P
IRAK1	NanoLuc	N	Jurkat	C
IRAK1	HiBiT	C	NCI-H1299	C
IRAK1	NanoLuc	N	THP-1	C
IRAK3	NanoLuc	N	THP-1	C
IRAK4	HiBiT	C	HeLa	C
IRAK4	HiBiT	C	K562 (LgBiT)	C
IRAK4	HiBiT	C	NCI-H1299	C
IRF5	HiBiT	N	THP-1	C
IRF7	HiBiT	N	THP-1	C
JAK1	NanoLuc	C	HEK293	C
JAK2	NanoLuc	C	TF-1	C
JAK2(V617F)	HiBiT	C	Hel 92.1.7	C
JAK2(V617F)	NanoLuc	C	Hel 92.1.7	C
KAT6A	HiBiT	N	T-47D	C
KDM2A	HiBiT	N	U-2 OS	P
KDM2A	HiBiT	N	HEK293 (LgBiT)	P
KLK3	HiBiT	C	22Rv1	C
KRAS (G12C)	HiBiT	N	MIA PaCa-2	C
KRAS(G12D)	HiBiT	N	AsPC-1	C
KRAS(G12D)	HiBiT	N	HEK293	C
KRAS(G12D)	HiBiT	N	PANC-1	C
KRAS(G12D)	HiBiT	N	SW 1990	C
KRAS(G12S)	HiBiT	N	A549	C
KRAS(G12V)	HiBiT	N	SW620	C
KRAS(G12V)	HiBiT	N	HEK293	C
KRAS(G13D)	HiBiT	N	NCI-H647	C
KRAS(WT)	HiBiT	N	HEK293	C
KRAS(WT)	HiBiT	N	NCI-H1299	C
LIG1	HiBiT	C	SK-OV-3	P
MAP2K1	HiBiT	N	NCI-H1299	C
MAP3K7	HiBiT-HaloTag	N	THP-1	C
MAP3K7	HiBiT-HaloTag	C	THP-1	C
MAP3K14	HiBiT-HaloTag	C	HeLa	C
MAP4K1 (HPK1)	NanoLuc	N	Jurkat	C
MAP4K1 (HPK1)	HiBiT	N	Jurkat	C/P
MAP4K1 (HPK1)	HiBiT-HaloTag	N	Jurkat	C
MAPK14	NanoLuc	C	HEK293	C
MAPT	HiBiT	N	IMR-32	C
MAPT (2N4R)	HiBiT	C	IMR-32	C
MARC1	HiBiT-HaloTag	C	HEK293	C
MET	HiBiT	C	A549	C
MET	HiBiT	C	HeLa	C
Myc (cMyc)	HiBiT	C	HEK293 (LgBiT)	C
MYH3	HiBiT	C	RD	C
NFE2L2 (NRF2)	HiBiT	N	A549	C/P
NFE2L2 (NRF2)	HiBiT	N	NCI-H460	C
NRAS(Q61R)	HiBiT	N	SW 1271	C
NRAS(WT)	HiBiT	N	HEK293	C
NTN4 (NETRIN-4)	HiBiT	C	DLD-1	P
PARP1	HiBiT	C	HeLa	P
PAX8	HiBiT	N	NIH:OVCAR-3	P
PBRM1	HiBiT	N	HEK293 (LgBiT)	C
PBRM1	HiBiT	C	HEK293 (LgBiT)	C
PIK3CA	HiBiT	N	T-47D	C
PIK3CA(E545K)	HiBiT	N	MCF7	C
PIK3CA(H1047R)	HiBiT	N	T-47D	C
PIK3C3	HiBiT	N	HEK293 (LgBiT)	C
PKMYT1	HiBiT	N	HEK293 (LgBiT)	P
PKMYT1	HiBiT	C	HEK293 (LgBiT)	P
PLA2R1	HiBiT	N	A431	C
PLK1	HiBiT	N	A549	P
PLK4	HiBiT	C	CHP-212	C
POLQ	HiBiT-HaloTag	C	DLD-1	P
POLQ	NanoLuc	C	DLD-1	C/P
POLQ	NanoLuc	N	DLD-1	P
PRMT5	HiBiT	N	A549	C
PRMT5	HiBiT	N	NCI-H1299	C
PTPN1	HiBiT	N	Jurkat (LgBiT)	C
PTPN2	HiBiT	N	Jurkat (LgBiT)	C
PTPN2 (isoform 2)	HiBiT	C	Jurkat (LgBiT)	C
PTPN22	HiBiT	N	Jurkat	C
PTPN6 (SHP1)	HiBiT	C	THP-1	C
RAF1 (cRAF)	HiBiT	C	A549	C/P
RAF1 (cRAF)	HiBiT	C	Hs 766T	C
RAF1 (cRAF)	HiBiT	C	Panc 08.13	C
RIPK1	HiBiT	N	A375	C
RIPK1	HiBiT	C	A375	C
RIPK2	HiBiT	C	THP-1	C
RUBCN	HiBiT	N	HEK293	C
RUBCN	HiBiT	C	HEK293 (LgBiT)	C
SALL4	HiBiT	N	SK-N-DZ	C
SETDB1	HiBiT	C	A375	C
SETDB1	HiBiT	N	A375	C
SKP2	HiBiT	C	22Rv1	C
SMAD2	HiBiT-HaloTag	N	THP-1	C
SMAD3	HiBiT	N	HeLa (LgBiT)	C
SMAD3	HiBiT-HaloTag	N	THP-1	C
SMARCA2	HiBiT	C	HeLa	C/P
SMARCA4	HiBiT	C	HeLa	C/P
SMARCA4	HiBiT	C	HeLa (LgBiT)	P
SOD2	HiBiT-HaloTag	C	T-47D	C
SPI1	HiBiT-HaloTag	N	THP-1	C
STAT3	HiBiT	C	HeLa (LgBiT)	C
STAT3-beta	HiBiT	C	HeLa (LgBiT)	C
STAT6	HiBiT	C	Jurkat	P
STAT6	HiBiT	C	Jurkat (LgBiT)	C
STC1	HiBiT-HaloTag	C	A549	C
SUCLA2	HiBiT	C	22Rv1	C
α-Synuclein (SNCA)	HiBiT	C	HeLa	C
TASL	HiBiT	C	THP-1	C
TEAD2	HiBiT	N	HEK293	C
TNFRSF1A	HiBiT	C	HeLa	P
TRIM63	HiBiT	N	C2C12	P
TREM2	HiBiT	N	THP-1	C
TREM2	HiBiT	C	THP-1	C
TREM2	HiBiT-HaloTag	C	THP-1	C
TYROBP (DAP12)	HiBiT	C	THP-1	C
TYROBP (DAP12)	HiBiT-HaloTag	C	THP-1	C
UBASH3A	HiBiT	C	Jurkat	P
UBASH3A	HiBiT	N	Jurkat	C
UBASH3B	HiBiT	C	Jurkat	C
USP14	HiBiT	C	HEK293 (LgBiT)	C/P
USP14	HiBiT	N	HEK293 (LgBiT)	P
WRN	HiBiT	N	HCT116	C
WRN	HiBiT	C	HEK293	C
XBP1	HiBiT	N	MCF7	C
XBP1s	HiBiT	C	MCF7	C
ZC3H12A	HiBiT	C	HeLa (LgBiT)	C
ZC3H12A	HiBiT	C	Jurkat (LgBiT)	C
ZC3H12A	HiBiT	N	Jurkat (LgBiT)	C

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CRISPRノックインタギングを利用したタンパク質存在量、内在化、制御のモニタリング

調節されたタンパク質存在量の決定

細胞内のタンパク質レベルは、細胞状態の変化によって自然に調整されており、治療戦略として意図的に操作される場合もあります。近年、狙ったタンパク質だけを分解する手法が開発され、これまで標的にできなかったタンパク質へのアプローチを可能にする新しい低分子モダリティとして脚光を浴びています。CRISPR / Cas9 ゲノム編集を利用して生物発光タグを内在的に挿入すると、特異的抗体を必要とすることなくタンパク質存在量を測定することができます。これらのアッセイは感度が高く、エンドポイントフォーマットまたは生細胞フォーマットで測定できるため、内在性の標的タンパク質レベルの詳細な分析が可能になります。

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ALSTEM, Inc. – Delivers custom iPSCs, CRISPR/Cas9-edited cell lines, and viral vectors, supporting research in regenerative medicine, disease modeling, drug discovery, and basic biology.

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