ReliaPrep™ DNA Clean-Up and Concentration System
Concentrate DNA from Large Volumes to ≤15µl
- Column-based method for rapid concentration and clean-up of DNA
- High binding capacity to accommodate large samples
- Small elution volumes provide highly concentrated samples and remove inhibitors
Catalog Number:
Size
Catalog Number: A2891
Catalog Number: A2892
Catalog Number: A2893
Prepare DNA Samples for Downstream Applications
The ReliaPrep™ DNA Clean-Up and Concentration System is designed to quickly concentrate and purify dilute DNA solutions, extract and purify DNA fragments of 100bp–10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) buffer, or to purify products directly from a PCR amplification. This membrane-based system can bind up to 60µg of DNA and concentrate as much as 300µl of dilute DNA, recovering isolated DNA fragments or PCR products in as little as 10 minutes, depending on the number of samples processed and the protocol used.
A single reagent stream is used for all three procedures, making the system both fast and easy. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, next-generation sequencing or in vitro transcription/translation.
The ReliaPrep™ DNA Clean-Up and Concentration System can be used with linear DNA fragments, supercoiled plasmid DNA or single-stranded linear or circular DNA.
Recovery and Concentration of DNA from Dilute Solution using ReliaPrep™ DNA Clean-Up and Concentration System
DNA (100bp DNA Ladder, Cat.# G2101) was diluted in triplicate into 100µl final volume of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Average yield and purity was 90.8%. Samples were further analyzed on an Agilent Bioanalyzer® 2100 using a DNA 1000 chip and protocol.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 1 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
Buffer B (BWB) |
A288A | 1 × 11.8ml | |
|
Membrane Binding Solution |
A930A | 1 × 4ml | View Product |
|
Elution Tubes |
Z200A | 2 × 5/pk | |
Nuclease-Free Water |
P119A-C | 1 × 1.25ml |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
Buffer B (BWB) |
A288A | 1 × 11.8ml | |
|
Membrane Binding Solution |
A930B | 1 × 20ml | View Product |
|
Elution Tubes |
Z200B | 2 × 25/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 1 × 50/pack | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
Buffer B (BWB) |
A288B | 1 × 58.8ml | |
|
Membrane Binding Solution |
A930B | 2 × 20ml | View Product |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
|
Collection Tubes |
A130B | 1 × 250/pk |
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