Wizard® SV Genomic DNA Purification System
Extracts DNA from Tissues or Cells
- Isolate DNA from cultured cells in 20 minutes
- Choice of spin or vacuum purification protocols
Catalog Number:
Size
Catalog Number: A2360
Catalog Number: A2361
Simple DNA Purification Method for Cells and Tissues
The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.
The Wizard® SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).
Sample Types Processed With Wizard® SV Genomic
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Peripheral blood mononuclear cells
|
Yes
|
Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11, 8699–705.
PubMed ID #16361556. | |
|
Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes
|
Yes
|
Morii, E. et al. (2004) Blood 104, 1656–61.
PubMed ID #15172970. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Mouse liver
|
Yes
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
| |
|
Mouse brain
|
Yes
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
| |
|
Mouse heart
|
Yes
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
| |
|
Mouse spleen
|
Yes
|
Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42.
PubMed ID #16498502. | |
|
Mouse ear
|
Yes
|
Du, Q. et al. (2006) FASEB J. 20, 1131–41.
PubMed ID #16770012. | |
|
Mouse skin
|
Yes
|
Basal cell carcinoma and noncancerous tissues were frozen at –80°C prior to DNA isolation.
Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14. PubMed ID #16818633. | |
|
Anopheles funestus mosquito carcass
|
Yes
|
Michel, A.P. et al. (2006) Genetics 173, 1389–95.
PubMed ID #16648581. | |
|
Human lung cancer biopsy
|
Yes
|
Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9.
PubMed ID #15837743. | |
|
Human distant normal colorectal mucosa and tumor tissue
|
Yes
|
Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7.
PubMed ID #15485994. | |
|
Human frozen thymic epithelial tumor samples
|
Yes
|
Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6.
PubMed ID #16762968. | |
|
Human pancreatic islet
|
Yes
|
Del Guerra, S. et al. (2005) Diabetes 54, 727–35.
PubMed ID #15734849. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Chicken retinospheroid cultures
|
Yes
|
Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24.
PubMed ID #16207942. | |
|
HT1080 human fibrosarcoma
|
Yes
|
Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction.
Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80. PubMed ID #16973543. | |
|
Monkey primary fibroblasts, virus-infected
|
Yes
|
Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated.
Blanchard, E. et al. (2006) J. Virol. 80, 6964–72. PubMed ID #16809302. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
E. coli K-12 derivative, MC4100
|
Yes
|
Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21.
PubMed ID #13129953. | |
|
Pseudomonas aeruginosa
|
Yes
|
Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903.
PubMed ID #15161855. | |
|
Bacillus subtilis
|
Yes
|
Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3.
PubMed ID #14973012. | |
|
Eleven Gram-negative bacterial species
|
Yes
|
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488. | |
|
Eleven Gram-positive bacterial species
|
Yes
|
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2mg/ml proteinase K was added (final concentration 0.4mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Snail cercariae
|
Yes
|
Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716. |
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
Column Wash Solution (CWA) |
A1311 | 1 × 185ml | View Product |
|
Nuclei Lysis Solution |
A7941 | 1 × 50ml | View Product |
|
RNase A Solution |
A797A | 1 × 250μl | View Product |
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
|
EDTA, 0.5M (pH 8.0) |
V423B | 1 × 30ml | View Product |
|
Wizard® SV Lysis Buffer |
Z3052 | 1 × 50ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | ||
|
Collection Tubes |
A130B | 1 × 250/pk | ||
Column Wash Solution (CWA) |
A1311 | 2 × 185ml | View Product | |
|
Nuclei Lysis Solution |
A7941 | 1 × 50ml | View Product | |
|
RNase A Solution |
A797C | 2 × 1ml | 4mg/ml | View Product |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product | |
|
EDTA, 0.5M (pH 8.0) |
V423B | 1 × 30ml | View Product | |
|
Wizard® SV Lysis Buffer |
Z3052 | 2 × 50ml | View Product |
Resources
その他
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関連製品
Vac-Man® Jr. Laboratory Vacuum Manifold
一度に 2サンプルから核酸を精製するためのデバイス
A7660, A3110
Vac-Man® Laboratory Vacuum Manifold
最大 20 サンプルを同時に処理できる迅速で効率的な核酸精製用吸引マニホールド
A7231
Proteinase K (凍結乾燥粉末)
広い切断特異性、尿素やSDS中でも安定であり未変性タンパク質を分解できる
V3021
QuantiFluor® ONE dsDNA System
DNA結合色素、DNAスタンダードとバッファーを含む、二本鎖DNAのシンプルな“添加と測定”による定量システム
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Nuclei Lysis Solution
Wizard®, Wizard® SVおよびWizard® SV 96 Genomic DNA Purification Kitのコンポーネントで、別途購入可能
A7941, A7943
Wizard® SV Lysis Buffer
細胞の溶解に用いるWizard® SVあるいはSV 96 Genomic DNA Purification Systemsのコンポーネント
Z3052
Column Wash Solution (CWA)
Wizard®, Wizard® SVおよびWizard® SV 96 Genomic DNA Purification Kitのコンポーネントで、別途購入可能
A1311, A1318
