Promoter-Driven Control Firefly Luciferase Vectors
Vectors That Are Co-transfected into Cells as Controls in Dual-Reporter Assays
- Co-transfect into cells where Renilla or NanoLuc® luciferase is the experimental reporter gene
- SV40, PGK and TK promoter options offer different expression levels suitable for most dual-reporter assays
Catalog Number:
Choose a promoter
Size
Catalog Number: E6681
Catalog Number: E5011
Catalog Number: E5061
プロモーター駆動ウミシイタケ 補正用ベクターは通常 Dual-Luciferase® または Dual-Glo® Reporter Assay Systemで使用する場合に実験用ホタルルシフェラーゼベクターとともにコトランスフェクションします。実験用のホタルベクターからの値が実験処理によって変動するのとは対照的に補正用のウミシイタケベクターからの活性レベルはほぼ不変である必要があります。
プロモーター駆動の pGL4.13 ホタルベクターは実験ベクターをウミシイタケにする状況で使用することができます。pGL4.50 および pGL4.51 は安定導入細胞作成のための
タグ付けや選択マーカーの提供の際に有用です。pGL4.50および pGL4.51ベクターは in vivo発光イメージングでの使用の際に細胞株のタグ付に理想的なベクターです。
感度の向上と生物学的関連性の強化:
- レポーター遺伝子発現向上: 哺乳動物細胞発現のための合成遺伝子のコドンの最適化
- バックグラウンドとアーティファクトの低減: 曖昧な調節エレメントや転写因子結合サイトを除去
- 応答性の改善: 不安定化ルシフェラーゼ遺伝子を用いたRapid Response™ 技術を利用可能
その他の利点:
- 柔軟な検出オプション: Choice of either 合成 luc2 (Photinus pyralis) または hRluc (Renilla reniformis) レポーター遺伝子から選択可能
- トランジェントからステイブルへの変更が容易: 哺乳動物細胞用選択マーカーを選択可能
- ベクター間の移動が簡便: 共通のマルチクローニングサイトおよびユニークなSfiIトランスファースキーム
- 転写調節
- ウイルス-細胞 相互作用
- 化合物スクリーニング
- 翻訳後修飾
- GPCR シグナリング
- 細胞内シグナリング
- プロモーター解析
Protocols & Applications Guide もご参照ください。
【ライセンス】pGL4ベクターシリーズ
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
Sequence Information
pGL4.13[luc2/SV40] Vector GenBank® Accession Number AY738225 and vector sequence text file.
pGL4.53[luc2/PGK] Vector GenBank® Accession Number KM359768 and vector sequence text file.
pGL4.54[luc2/TK] Vector GenBank® Accession Number KM359769 and vector sequence text file.
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.13[luc2/SV40] Vector |
E668A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.53[luc2/PGK] Vector | E501A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
| pGL4.54[luc2/TK] Vector | E506A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
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動物個体の発光イメージングに使用する高発現ルシフェラーゼレポーターベクタ^
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pGL4.10[luc2]、pGL4.11[luc2P]、pGL4.12[luc2CP]ベクターはそれぞれ安定性の異なるルシフェラーゼタンパク質をコードし、遺伝子発現転写調節領域の研究に使用できます。
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Promoter-Driven Control NanoLuc® Luciferase Vectors
NanoLuc® (Nluc) control vectorはdual-reporter assayにおいて補正用コントロールとして実験レポーターベクターと細胞にコトランスフェクションします。
N1441, N1501, N1091
関連製品
Dual-Luciferase® Reporter Assay System
単一サンプルでのホタル、ウミシイタケルシフェラーゼ活性の連続測定。インジェクター2本必要。
E1910, E1960, E1980
Dual-Glo® Luciferase Assay System
単一サンプルでのホタル、ウミシイタケルシフェラーゼ活性の連続測定、シグナル半減期2時間。インジェクター不要。
E2920, E2940, E2980
Nano-Glo® Dual-Luciferase Reporter Assay System
シングルサンプル中のホタルおよび NanoLuc® ルシフェラーゼ活性を高感度に検出
N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551
Bright-Glo™ Luciferase Assay System
ロボットを用いた連続処理にも使える高感度レポーターアッセイ
E2610, E2620, E2650