- 便利: 希釈・混和せずに、そのまま使用可能
- 安定: 室温で1年間安定
- ウェスタンブロッティング
- ドットブロッティング
- Cedeno-Laurent, F. et al. (2012) Galectin-1 triggers an immunoregulatory signature in Th cells functionally defined by IL-10 expression. J. Immunol. 188, 3127–37.
- Petrova, N.S. et al. (2012) Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group. Nucleic Acids Res. 40, 2330–44.
- To, W.S. and Midwood, K.S. (2011) Identification of novel and distinct binding sites within Tenascin-C for soluble and fibrillar fibronectin. J. Biol. Chem. 286, 14881–91.
The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng). Primary antibody (monoclonal) used at 1:5,000; Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).
Protocols
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