S3841_Western-Blue--Stabilized-Sub-for-AlkPhos_3

  • Ready-to-use formulation that does not require dilution or reagent mixing
  • As sensitive as other commercially available BCIP/NBT formulations and reagents
  • Stable for one year at room temperature

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Catalog Number: S3841

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ウエスタンブロットやイムノスクリーニングに使用できる安定なアルカリホスファターゼの基質です。Western Blue®Substrateは希釈せずにそのまま使用します。この基質は5-bromo-4-chloro-3-indolyl-1-phosphate(BCIP)とnitro blue tetrazolium(NBT)を専用の安定化バッファーに溶解しています。アルカリホスファターゼにより、退色しにくい黒紫色のスポットがメンブレン上に発色されます。Western Blue®Stabilized Substrateは、調製が必要なBCIP/NBT試薬と同等の検出感度を持ち、室温で1年間は安定です。

  • 便利: 希釈・混和せずに、そのまま使用可能
  • 安定: 室温で1年間安定

  • ウェスタンブロッティング
  • ドットブロッティング

  1. Cedeno-Laurent, F. et al. (2012) Galectin-1 triggers an immunoregulatory signature in Th cells functionally defined by IL-10 expression. J. Immunol. 188, 3127–37.
  2. Petrova, N.S. et al. (2012) Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group. Nucleic Acids Res. 40, 2330–44.
  3. To, W.S. and Midwood, K.S. (2011) Identification of novel and distinct binding sites within Tenascin-C for soluble and fibrillar fibronectin. J. Biol. Chem. 286, 14881–91.
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng). Primary antibody (monoclonal) used at 1:5,000; Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).

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For Research Use Only. Not for Use in Diagnostic Procedures.

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