Transfection Carrier DNA is a plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection without reducing the overall amount of DNA. Diluting reporter DNA into Transfection Carrier DNA results in lowered expression of the protein of interest, overcoming artifacts due to overexpression. This is especially relevant when strong, constitutive promoters (e.g., CMV) are used. Depending on the application, the ratio of reporter to Transfection Carrier DNA may require optimization.
This product is made from pGEM®-3Zf(–) Vector but has been purified using a method that results in low endotoxin carryover, making it more suitable for use in transfecting mammalian cells.