CaspACE™ FITC-VAD-FMK In Situ Markerは細胞透過性のフルオロチオシアネート（FITC）標識カスパーゼ阻害剤VAD-FMKです。この構造は細胞への移動を容易にし、透過した阻害剤は活性化カスパーゼに不可逆的に結合します。FITC標識により細胞内におけるカスパーゼ活性の定量を直接的にワンステップで行うことができます。本製品は蛍光検出によりカスパーゼ活性をin situでモニタリングすることができます。FITC-VAD-FMKは、DMSOに溶解された5mM溶液として供給されます。Anti-Fas処理したJurkat細胞に使用する場合の目安は10µM程度です。
CaspACE™ FITC-VAD-FMK In Situ Marker is a fluorescent analog of the pan caspase inhibitor Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone). The fluorescein isothiocyanate (FITC) group has been substituted for the carbobenzoxy (Z) N-terminal blocking group to create the fluorescent apoptosis marker. This structure allows delivery of the inhibitor into the cell where it irreversibly binds to activated caspases. The FITC label allows for a single-reagent addition to assay for caspase activity in situ. The FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in situ monitoring of caspase activity by fluorescence detection. The suggested concentration for use in anti-Fas-treated Jurkat cell culture is 10μM.
CaspACE™ FITC-VAD-FMK In Situ Marker is a fluorescent analog of the pan caspase inhibitor Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone). The fluorescein isothiocyanate (FITC) group has been substituted for the carbobenzoxy (Z) N-terminal blocking group to create the fluorescent apoptosis marker. This structure allows delivery of the inhibitor into the cell where it irreversibly binds to activated caspases. The FITC label allows for a single-reagent addition to assay for caspase activity in situ. The FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in situ monitoring of caspase activity by fluorescence detection. The suggested concentration for use in anti-Fas-treated Jurkat cell culture is 10μM.
CaspACE™ FITC-VAD-FMK In Situ Marker is a fluorescent analog of the pan caspase inhibitor Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone). The fluorescein isothiocyanate (FITC) group has been substituted for the carbobenzoxy (Z) N-terminal blocking group to create the fluorescent apoptosis marker. This structure allows delivery of the inhibitor into the cell where it irreversibly binds to activated caspases. The FITC label allows for a single-reagent addition to assay for caspase activity in situ. The FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in situ monitoring of caspase activity by fluorescence detection. The suggested concentration for use in anti-Fas-treated Jurkat cell culture is 10μM.
CaspACE™ FITC-VAD-FMK In Situ Marker is a fluorescent analog of the pan caspase inhibitor Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone). The fluorescein isothiocyanate (FITC) group has been substituted for the carbobenzoxy (Z) N-terminal blocking group to create the fluorescent apoptosis marker. This structure allows delivery of the inhibitor into the cell where it irreversibly binds to activated caspases. The FITC label allows for a single-reagent addition to assay for caspase activity in situ. The FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in situ monitoring of caspase activity by fluorescence detection. The suggested concentration for use in anti-Fas-treated Jurkat cell culture is 10μM.
CaspACE™ FITC-VAD-FMK In Situ Marker is a fluorescent analog of the pan caspase inhibitor Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone). The fluorescein isothiocyanate (FITC) group has been substituted for the carbobenzoxy (Z) N-terminal blocking group to create the fluorescent apoptosis marker. This structure allows delivery of the inhibitor into the cell where it irreversibly binds to activated caspases. The FITC label allows for a single-reagent addition to assay for caspase activity in situ. The FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in situ monitoring of caspase activity by fluorescence detection. The suggested concentration for use in anti-Fas-treated Jurkat cell culture is 10μM.
• 簡単：FITC-VAD-FMKを添加→インキュベート→洗浄→蛍光観察
• 汎用性：蛍光顕微鏡、フローサイトメトリーでアポトーシス細胞を検出。ハイスループットなアプリケーションにも対応
• 迅速：培養細胞に1種類の試薬を加えるだけで、細胞抽出液の調製や長時間のインキュベーションが不要
• 信頼性：バッチによりばらつきのあるAnnexin Vに比べ合成ペプチドは安定した品質
• 細胞透過性：細胞の内外を自由に移動でき、アポトーシス細胞にのみ定着
Protocols & Applications Guide もご参照ください。
【Q&A】
CaspACE FITC-VAD-FMK In Situ Marker
【アプリケーション】フローサイトメトリーを用いたアポトーシス細胞の検出 CaspACETM FITC-VAD-FMK In Situ Markerを用いたアポトーシス研究
- Yang, T. et al. (2002) Can. Res. 62, 2583–91.
- Elbaz, M. et al. (2002) Cell Death Diff. 9, 726–33.
- Madeo, F. et al. (2002) Mol. Cell 9, 911–7.
- Haiyan, Q. et al. (2003) J. Biol. Chem. 278, 15136–41.