Lumit® IL-12 p70 (Human) Immunoassay
No-Wash Immunoassay for IL-12
- Cell-based assay with fast add-and-read protocol
- Luminescent detection with any plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W1850
Catalog Number: W1851
Catalog Number: W1852
Catalog Number: W185A-C
リン酸化または総タンパク質の高速定量
Lumit™ Immunoassay Cellular System は、細胞溶解液に含まれる標的分子を直接測定可能な洗浄のいらない生物発光イムノアッセイです。このアッセイは、適切な一次抗体（お客様提供）が利用可能であれば、あらゆるリン酸化タンパク質、総タンパク質あるいは低分子を検出できるように適合させることができます。
ご参考いただくために、注目度の高いターゲットについて市販の一次抗体を用いて Lumit™ イムノアッセイの評価を行いました。以下のアプリケーションノートには、試用した各一次抗体用のプロトコル、サンプルデータ、最適化条件、および注文情報が記載されされています。
How the Lumit® IL-12 Immunoassay Works
Primary antibodies to IL-12 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-12, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-12 levels. The assay is specific for the IL-12p70 (A/B heterodimer); it is effective for detection of IL-12p70 in presence of 100-fold excess of either IL-12B subunit, IL-12p80 or IL-23.
Simple One-Plate Protocol Requires No Wash Steps
Lumit™ イムノアッセイ vs. ウェスタンブロッティング
|
Specification |
Lumit® IL-12 (Human) Immunoassay |
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Dynamic Range |
18.2–25,000pg/ml |
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|
Limit of Detection (LOD) |
10.4pg/ml (3 SD above background) |
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Minimal Detectable Dose (MDD) |
7.1pg/ml (2 SD above background) |
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Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
High-Throughput Screening Applications
Optimizing the Lumit® IL-6 Immunoassay for High-Throughput Drug Screening
In this webinar, we share key considerations for scaling Lumit® Immunoassays from a single 96-well plate to many 364-well plates and offer tips for optimizing Lumit® Immunoassays for HTS applications. You will also hear Galapagos scientist Dr. Nick Verstraeten share how his team used the Lumit® IL-6 Immunoassay in a screening campaign for antiviral drugs.
Lumit® Cytokine Assay Automation for High-Throughput Screening
In this poster, see miniaturization and automation data for Lumit® cytokine assays.
Sensitive Detection of IL-12 p70 Released from Human PBMCs
Human PBMCs (100,000 cells/well) were pretreated with IFN-ɣ and then stimulated with increasing concentrations of LPS. After treatment, Lumit® IL-12 Immunoassay was used to quantify IL-12 release.
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml |
Anti-hIL-12 mAb-LgBiT, 500X |
W157A | 1 × 30μl |
Anti-hIL-12 mAb-SmBiT, 500X |
W186A | 1 × 30μl |
Human IL-12 Standard |
W185A | 1 × 25μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml |
Anti-hIL-12 mAb-LgBiT, 500X |
W157B | 1 × 300μl |
Anti-hIL-12 mAb-SmBiT, 500X |
W186B | 1 × 300μl |
Human IL-12 Standard |
W185A | 1 × 25μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml |
Anti-hIL-12 mAb-LgBiT, 500X |
W157A | 5 × 30μl |
Anti-hIL-12 mAb-SmBiT, 500X |
W186A | 5 × 30μl |
Human IL-12 Standard |
W185A | 1 × 25μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Human IL-12 Standard |
W185A | 1 × 25μl |
Resources
技術記事
技術ポスター
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