Lumit® IL-4 (Human) Immunoassay
No-Wash Immunoassay for IL-4 Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6060
Catalog Number: W6062
Catalog Number: W6061
Catalog Number: W125A-C
面倒な洗浄ステップが不要な放出サイトカイン定量イムノアッセイ
Lumit™ IL-4 Immunoassay は、洗浄不要でシンプルなプロトコルを用いて細胞培養サンプル中の放出 IL-4を定量的に測定します。サンプルに標識抗体を加え、検出試薬を加え、標準的なプレート測定型ルミノメーターを用いて発光シグナルを読み取るだけです。全プロトコルは70分以内に完了します。 このアッセイは、培養中の細胞や、別のアッセイプレートに移した培養液をサンプルとして直接使用することができます。
How the Lumit® IL-4 Immunoassay Works
Primary antibodies to IL-4 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-4, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-4 levels.
Simple One-Plate Protocol, No Wash Steps
Lumit™ 検出法の広いダイナミックレンジ
Lumit™ 検出のピコモルからナノモルのリニアレンジは、ほとんどの細胞モデルで予想される濃度をカバー。
|
Assay |
IL-4 Human Immunoassay |
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Limit of Detection (LOD) |
6.7pg/ml (3 SD above background) |
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|
Dynamic Range |
18.2pg/ml – 25ng/ml |
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Minimal Detectable Dose (MDD) |
4.5pg/ml (2 SD above background) |
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Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
Sensitive Quantitation of Low-Level IL-4 Release
When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit® IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.
Same-Well Multiplexing with Viability and Cytotoxicity Assays
Human T helper type 2 (Th2) cells were plated in 96-well plate format, primed overnight with hIL-2 and treated with increasing concentration of cell stimulation cocktail (CSC) for 24 hours. Following treatment, CellTox™ Green Cytotoxicity Assay and the Lumit® IL-4 (Human) Immunoassay reagents were added to determine cytotoxicity and IL-4 release. CellTiter-Glo® Cell Viability Assay reagent was added to determine cell viability. A dose-dependent increase in IL-4 was observed, with some degree of increased cytotoxicity and decreased cell viability.
Explore Additional Cytokine Detection Assays
We offer a growing portfolio of assays to detect cytokines in cell culture samples.
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For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Anti-hIL-4 mAb-SmBiT, 500X |
W126A | 1 × 30μl | |
Anti-hIL-4 mAb-LgBiT, 500X |
W127A | 1 × 30μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Anti-hIL-4 mAb-SmBiT, 500X |
W126A | 5 × 30μl | |
Anti-hIL-4 mAb-LgBiT, 500X |
W127A | 5 × 30μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Anti-hIL-4 mAb-SmBiT, 500X |
W126B | 1 × 300μl | |
Anti-hIL-4 mAb-LgBiT, 500X |
W127B | 1 × 300μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Resources
その他
- Rapid and Sensitive Determination of Cytokine Release from Cells Without Sample Transfer
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
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