Lumit® IL-4 (Human) Immunoassay

面倒な洗浄ステップが不要な放出サイトカイン定量イムノアッセイ

Lumit™ IL-4 Immunoassay は、洗浄不要でシンプルなプロトコルを用いて細胞培養サンプル中の放出 IL-4を定量的に測定します。サンプルに標識抗体を加え、検出試薬を加え、標準的なプレート測定型ルミノメーターを用いて発光シグナルを読み取るだけです。全プロトコルは70分以内に完了します。このアッセイは、培養中の細胞や、別のアッセイプレートに移した培養液をサンプルとして直接使用することができます。

How the Lumit® IL-4 Immunoassay Works

Primary antibodies to IL-4 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-4, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-4 levels.

Simple One-Plate Protocol, No Wash Steps

Lumit™ 検出法の広いダイナミックレンジ

Lumit™ 検出のピコモルからナノモルのリニアレンジは、ほとんどの細胞モデルで予想される濃度をカバー。

Assay	IL-4 Human Immunoassay
Limit of Detection (LOD)	6.7pg/ml (3 SD above background)
Dynamic Range	18.2pg/ml – 25ng/ml
Minimal Detectable Dose (MDD)	4.5pg/ml (2 SD above background)
Assay Time	70 minutes
Sample Type	Cell culture supernatants

SD=Standard Deviation

Sensitive Quantitation of Low-Level IL-4 Release

Quantitation of low-level IL-4 release with the lumit IL4 assay. — When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit® IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.

Same-Well Multiplexing with Viability and Cytotoxicity Assays

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

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For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

プロトコール

Complete Protocol

Lumit® IL-4 (Human) Immunoassay Technical ManualPDF (1935 KB) – English

選択製品の構成品内容

Item	Part #	Size	Concentration
Lumit® Detection Substrate B	VB405A	1 × 160μl
Lumit® Detection Buffer B	VB406A	1 × 3.2ml
Human IL-4 Standard	W125A	1 × 25μl	10μg/ml
Anti-hIL-4 mAb-SmBiT, 500X	W126A	1 × 30μl
Anti-hIL-4 mAb-LgBiT, 500X	W127A	1 × 30μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

パテントおよび免責条項

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.

選択製品の構成品内容

Item	Part #	Size	Concentration
Lumit® Detection Substrate B	VB405A	5 × 160μl
Lumit® Detection Buffer B	VB406A	5 × 3.2ml
Human IL-4 Standard	W125A	1 × 25μl	10μg/ml
Anti-hIL-4 mAb-SmBiT, 500X	W126A	5 × 30μl
Anti-hIL-4 mAb-LgBiT, 500X	W127A	5 × 30μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

パテントおよび免責条項

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.

選択製品の構成品内容

Item	Part #	Size	Concentration
Lumit® Detection Substrate B	VB405B	1 × 1.25ml
Lumit® Detection Buffer B	VB406B	1 × 25ml
Human IL-4 Standard	W125A	1 × 25μl	10μg/ml
Anti-hIL-4 mAb-SmBiT, 500X	W126B	1 × 300μl
Anti-hIL-4 mAb-LgBiT, 500X	W127B	1 × 300μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

パテントおよび免責条項

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.

選択製品の構成品内容

Item	Part #	Size	Concentration
Human IL-4 Standard	W125A	1 × 25μl	10μg/ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

技術資料

技術記事

Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover

Lumit® IL-4 (Human) Immunoassay

Part Numbers: W6060, W6062, W6061, W125A-C

洗浄不要の IL-4 検出用イムノアッセイ

面倒な洗浄ステップが不要な放出サイトカイン定量イムノアッセイ

How the Lumit® IL-4 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ 検出法の広いダイナミックレンジ

Sensitive Quantitation of Low-Level IL-4 Release

Same-Well Multiplexing with Viability and Cytotoxicity Assays

Explore Additional Cytokine Detection Assays

プロトコール

Complete Protocol

製品仕様

選択製品の構成品内容

SDS

分析証明書

使用制限

保存条件

パテントおよび免責条項

製品仕様

選択製品の構成品内容

SDS

分析証明書

使用制限

保存条件

パテントおよび免責条項

製品仕様

選択製品の構成品内容

SDS

分析証明書

使用制限

保存条件

パテントおよび免責条項

製品仕様

選択製品の構成品内容

SDS

分析証明書

使用制限

保存条件

技術資料

技術記事

その他

類似製品

Lumit® IL-1β Human/Mouse Immunoassay

Lumit® IL-2 (Human) Immunoassay

Lumit® IL-10 (Human) Immunoassay

Lumit® HMGB1 Human/Mouse Immunoassay

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GloMax® Discover System

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