DDR2 Kinase Enzyme System
Easily Screen and Profile DDR2 Kinase Inhibitors
- Includes kinase, substrate and reaction buffer
- Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
Catalog Number:
Size
Additional options
Catalog Number: V4058
Catalog Number: V6391
Catalog Number: V4059
Convenient, Scalable Kinase Profiling
Kinase Enzyme System contains:
- Kinase: DDR2, 10μg (Human, recombinant; amino acids 467–end). MW: ~70kDa.
- Substrate: Axltide (CKKSRGDYMTMQIG); derived from mouse insulin receptor substrate 1 (amino acids 979–989).
- Other: Reaction Buffer, DTT, MnCl2.
Human Kinase Enzyme Systems and Applications.
- Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
- Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
- Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
- Karn, T. et al. (1993) Structure, expression and chromosomal mapping of TKT from man and mouse: a new subclass of receptor tyrosine kinases with a factor VIII-like domain. Oncogene 8, 3433–40.
- Ferri, N. et al. (2004) Role of discoidin domain receptors 1 and 2 in human smooth muscle cell-mediated collagen remodeling: potential implications in atherosclerosis and lymphangioleiomyomatosis. Am J Pathol. 164, 1575–85.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
| DDR2 Kinase Enzyme System | V4058 | 1 × 10μg |
|
ADP-Glo™ Kinase Assay |
V9101 | 1 × 1,000 assays |
U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
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