すべてのKinase Enzyme Systemsのリスト
Kinase Enzyme Systemは上記のキナーゼ反応に必要な酵素および基質などのセットです。ADP-Glo™ Kinase Assayとのセット品もあり、高感度な発光法によりキナーゼ反応後に生じるADPを測定する
ADP-Glo™ Kinase Assayで簡便にキナーゼ活性を測定することができます。
Kinase Enzyme System contains:
- Kinase: HIPK1, 10μg (Human, recombinant; amino acids 156–555). MW: ~71kDa.
- Substrate: Native Swine Myelin Basic Protein (MBP).
- Other: Reaction Buffer, DTT.
HIPK1 NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
- Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
- Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
- Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
【Kinase Enzyme Systemデータシート＆アプリケーションノート】日本語
【Ｑ＆Ａ】ADP-Glo
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
- Ecsedy, J.A. et al. (2003) Homeodomain-interacting protein kinase 1 modulates Daxx localization, phosphorylation, and transcriptional activity. Mol Cell Biol. 23, 950–60.
- Kondo, S. et al. (2003) Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1). Proc Natl Acad Sci USA 100, 5431–6.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.