VRK2 Kinase Enzyme System
Easily Screen and Profile VRK2 Kinase Inhibitors
- Includes kinase, substrate and reaction buffer
- Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
Catalog Number:
Size
Additional options
Catalog Number: V4494
Catalog Number: V6570
Catalog Number: V4495
Convenient, Scalable Kinase Profiling
Kinase Enzyme System contains:
- Kinase: VRK2, 10μg (Human, recombinant; amino acids 1–375). MW: ~66kDa.
- Substrate: Native Casein Protein was purified from bovine milk.
- Other: Reaction Buffer, DTT, MnCl2.
Human Kinase Enzyme Systems and Applications.
- Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
- Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
- Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
【Kinase Enzyme Systemデータシート＆アプリケーションノート】日本語
【Ｑ＆Ａ】ADP-Glo
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
- Nezu, J. et al. (1997) Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase. Genomics 45, 327–31.
- Li, L.-Y. et al. (2001) The cellular protein PRA1 modulates the anti-apoptotic activity of Epstein-Barr virus BHRF1, a homologue of Bcl-2, through direct interaction. J Biol Chem 276, 27354–62.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
| VRK2 Kinase Enzyme System | V4494 | 1 × 10μg |
|
ADP-Glo™ Kinase Assay |
V9101 | 1 × 1,000 assays |
U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
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