Cell-Permeable Kinase NanoBRET® TE Tracers
Measure Cellular Affinity of Compounds for Kinase Targets in Live Cells
- Part of NanoBRET® Target Enhancement (TE) Intracellular Kinase Assays
- NanoBRET® TE Tracer products increase flexibility and modularity of NanoBRET® TE Kinase Assays
- Use with Intracellular TE Nano-Glo® substrate/inhibitor and NanoLuc®-kinase fusion vectors
Catalog Number:
Choose a tracer
Size
Catalog Number: N2602
Catalog Number: N2482
Catalog Number: N2492
Catalog Number: N2622
Catalog Number: N2632
Catalog Number: N2642
Catalog Number: N2652
Catalog Number: NF1011
生細胞内でのキナーゼ-リガンド親和性を測定
NanoBRET™ Tracer は、標的タンパク質に結合する低分子リガンドあるいは阻害剤が蛍光物質と共有結合した構造を有しています。いくつかの NanoBRET™ Tracerは、NanoBRET™ TE Intracellular Kinase Assay system の一部として開発されました。このシステムでは、生物発光共鳴エネルギー転移（BRET）ベースの方法を使用してインタクトな細胞内における特定の標的キナーゼと化合物の結合性を測定します。
具体的には、NanoBRET™ TE Kinase Assay により、細胞内のキナーゼ-NanoLuc®ルシフェラーゼ融合タンパク質に可逆的に結合した細胞透過性の NanoBRET™ Tracerを競合置換することにより、披験化合物の細胞内での見かけの親和性を分析することができます。 BRET アッセイではキナーゼ-NanoLuc® 融合タンパク質はエネルギードナーとして、蛍光 NanoBRET™ TE Tracer はエネルギーアクセプターとして機能します。
NanoBRET™ TE Tracer は、個別に購入することも、NanoBRET™ TE Intracellular Kinase Assay の一部として購入することもできます。各NanoBRET™ TE Kinase Assay は、NanoBRET™ Tracer、NanoBRET™ Nano-Glo® 基質、細胞外 NanoLuc® 阻害剤、トレーサー希釈バッファーなど、NanoBRET™検出に必要な試薬を提供します。 NanoBRET™ TE Tracer は、新しいキナーゼを研究したり、代替のトレーサーをテストしたりする柔軟性を提供します。
特定のキナーゼ-NanoLuc® 融合ベクターも 個別に入手可能です。選択したキナーゼ-NanoLuc®融合タンパク質に対して推奨される NanoBRET™ Tracer とその濃度は、 こちら のアプリケーションノートに記載されています。
NanoBRET™ Kinase Target Engagement についての詳細はこちら
テクノロジーについてProtocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-3 |
N260B | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-5 |
N248B | 1 × 550μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-4 |
N249B | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-8 |
N262B | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-9 |
N263B | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-10 |
N264B | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-11 |
N265B | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-12 |
NF101A | 1 × 300μl | 400μM |
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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関連製品
Intracellular TE Nano-Glo® Substrate/Inhibitor
NanoLuc®-HaloTag®融合タンパク質をコードし、効率的なエネルギー転移を確実にするためにNanoLuc®ドナーとHaloTag®アクセプタータンパク質がつながれています。
N2162, N2160, N2161
NanoBRET® Target Engagement Kinase Assays
キナーゼ - リガンド親和性およびレジデンスタイム（滞留時間）を直接的に定量測定する生細胞でのターゲットエンゲ―ジメントアッセイ
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor
An alternative NanoLuc® substrate and extracellular inhibitor for use in NanoBRET® Target Engagement Intracellular Assays that will have extended kinetic monitoring.
N2200, N2201
