Intracellular TE Nano-Glo® Substrate/Inhibitor
For use with NanoBRET® Target Engagement Intracellular Assays
- Supplies the NanoBRET® Nano-Glo® Substrate and Extracellular NanoLuc® Inhibitor
- Optimal for experiments ≤2 hours
- Ensures measurements originate from inside intact cells
Catalog Number:
Size
Catalog Number: N2162
Catalog Number: N2160
Catalog Number: N2161
Intracellular TE Nano-Glo® Substrate/Inhibitor は Intracellular NanoBRET™ Target Engagement (TE) assay で使用する別売品です。 本製品には NanoBRET™ Nano-Glo® substrate と Extracellular NanoLuc® Inhibitor の2つの構成品が含まれています。NanoBRET™ Nano-Glo® Substrate は NanoBRET™ Assay シリーズでドナーシグナルを生じさせる NanoLuc® ルシフェラーゼ の基質です。Extracellular NanoLuc® Inhibitor は細胞外のあらゆるNanoLuc®シグナルを抑制するために使用します。この阻害剤によってインタクトな細胞内からの NanoBRET™ シグナルを測定することができます。
この Intracellular TE Nano-Glo® Substrate/Inhibitor は以下のシステムの構成品として使用することができます :
- NanoBRET™ Target Engagement HDAC Assays
- NanoBRET™ Target Engagement BET BRD Assays
- NanoBRET™ Target Engagement Kinase Assays
さらに、 Intracellular TE Nano-Glo® Substrate/Inhibitor は NanoBRET™ Dyes ( Custom Assay Services より入手可能) を含む Intracellular NanoBRET™ Target Engagement Tracer とともに使用することができます。詳細については お問合せください。
Measure Kinase-Compound Affinity in Live Cells
Learn More about NanoBRET® Kinase Target EngagementSpecifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157A | 1 × 50μl | View Product |
Extracellular NanoLuc® Inhibitor |
N235A | 1 × 17μl | View Product |
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157C | 1 × 330μl | View Product |
Extracellular NanoLuc® Inhibitor |
N235B | 1 × 110μl | View Product |
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157D | 1 × 3.3ml | View Product |
Extracellular NanoLuc® Inhibitor |
N235C | 1 × 1.1ml | View Product |
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Related Products
関連製品
NanoBRET® Target Engagement Kinase Assays
キナーゼ - リガンド親和性およびレジデンスタイム（滞留時間）を直接的に定量測定する生細胞でのターゲットエンゲ―ジメントアッセイ
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
NanoBRET® Target Engagement BET BRD Assays
生細胞内で BET ブロモドメイン と テスト化合物の親和性およびレジデンスタイムを直接的に測定
N2131, N2180, N2130, N2140
NanoBRET® Target Engagement HDAC Assays
生細胞における HDAC と テスト化合物の親和性およびレジデンスタイムの直接的な測定
N2080, N2081, N2170, N2090
